Recombinant Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2851] to SLP76 (phospho Y113)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SLP76 (phospho Y113) antibody [EPR2851]
See all SLP76 primary antibodies -
Description
Rabbit monoclonal [EPR2851] to SLP76 (phospho Y113) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat cytoplasmic lysate treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2851 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
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Notes |
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WB
1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa). |
Target
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Function
Involved in T-cell antigen receptor mediated signaling. -
Tissue specificity
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. -
Sequence similarities
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain. -
Domain
The SH2 domain mediates interaction with SHB. -
Post-translational
modificationsPhosphorylated after T-cell receptor activation by ZAP-70. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3937 Human
- Omim: 601603 Human
- SwissProt: Q13094 Human
- Unigene: 304475 Human
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Alternative names
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
see all
Images
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All lanes : Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990) at 1/10000 dilution
Lane 1 : Jurkat cell lysate - untreated
Lane 2 : Jurkat cell lysate - treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab75990 has been referenced in 3 publications.
- Hoshino A et al. Dysregulation of Epstein-Barr Virus Infection in Hypomorphic ZAP70 Mutation. J Infect Dis 218:825-834 (2018). PubMed: 29684201
- Cimo AM et al. CD25 and CD69 induction by a4ß1 outside-in signalling requires TCR early signalling complex proteins. Biochem J 454:109-21 (2013). WB ; Human . PubMed: 23758320
- Köhler K et al. Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells. PLoS One 5:e15374 (2010). WB ; Human . PubMed: 21179506