Anti-PKC theta/PRKCQ antibody [M217] (ab76197)
Key features and details
- Mouse monoclonal [M217] to PKC theta/PRKCQ
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-PKC theta/PRKCQ antibody [M217]
See all PKC theta/PRKCQ primary antibodies -
Description
Mouse monoclonal [M217] to PKC theta/PRKCQ -
Host species
Mouse -
Specificity
ab76197 is not predicted to react with other PKC family members due to low sequence homology. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Jurkat whole cell lysate (ab7899). ICC/IF: PC-12 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M217 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100.
|
|
WB |
1/1000. Predicted molecular weight: 82 kDa.
|
Notes |
---|
ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 82 kDa. |
Target
-
Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Skeletal muscle, megakaryoblastic cells and platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
-
Database links
- Entrez Gene: 5588 Human
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
- Unigene: 225125 Rat
-
Alternative names
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
Images
-
Lane 1 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/250 dilution
Lane 2 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/1000 dilution
Lane 1 : Jurkat cell lysate.
Lane 2 : Jurkat cell lysate
Predicted band size: 82 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted? -
NGF-differentiated PC-12 cells (rat adrenal gland pheochromocytoma cell line) stained for PKC theta/PRKCQ using ab76197 at 1/100 dilution in ICC/IF. Secondary antibody is a Cy3 conjugate.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab76197 has been referenced in 1 publication.
- Zhou LJ et al. Toxoplasma gondii SAG1 targeting host cell S100A6 for parasite invasion and host immunity. iScience 24:103514 (2021). PubMed: 34950858