Recombinant Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Synapsin I (phospho S9) antibody [EP2162Y]
See all Synapsin I primary antibodies -
Description
Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat brain tissue lysates, untreated or treated with AP.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2162Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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Dot blot |
1/1000.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
Dot blot
1/1000. |
Target
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Function
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
Involvement in disease
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
Sequence similarities
Belongs to the synapsin family. -
Post-translational
modificationsSubstrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
- SwissProt: P09951 Rat
- Unigene: 225936 Human
see all -
Alternative names
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
Images
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution
Lanes 1 & 3 : Human brain whole cell lysate - untreated
Lanes 2 & 4 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?Exposure time:
Lanes 1 and 2: 1 second.
Lanes 3 and 4: 15 seconds.
Blocking and dilution buffer:
Lanes 1 and 2: 5% NFDM/TBST.
Lanes 3 and 4: 2% BSA/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/5000 dilution
Lane 1 : Human brain whole cell lysate - untreated
Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/200 dilution
Lane 1 : Mouse brain whole cell lysate - untreated
Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution
Lane 1 : Rat brain whole cell lysate - untreated
Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and dilution buffer: 2% BSA/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)
Lane 1 : Human brain tissue lysate, untreated
Lane 2 : Human brain tissue lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa -
Dot blot analysis of Synapsin I (pS9) phospho peptide (lane 1) and Synapsin I non-phospho peptide (lane 2) labelling Synapsin I (pS9) with ab76260 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab76260 has been referenced in 6 publications.
- Shi HJ et al. Requirement of hippocampal DG nNOS-CAPON dissociation for the anxiolytic and antidepressant effects of fluoxetine. Theranostics 12:3656-3675 (2022). PubMed: 35664081
- Yan P et al. Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons. Cell Death Dis 13:786 (2022). PubMed: 36097267
- Dou Y et al. Neurotransmitter-stimulated neuron-derived sEVs have opposite effects on amyloid β-induced neuronal damage. J Nanobiotechnology 19:324 (2021). PubMed: 34654438
- Campos CA & Ritter RC NMDA-type glutamate receptors participate in reduction of food intake following hindbrain melanocortin receptor activation. Am J Physiol Regul Integr Comp Physiol 308:R1-9 (2015). PubMed: 25394828
- Campos CA et al. Central vagal afferent endings mediate reduction of food intake by melanocortin-3/4 receptor agonist. J Neurosci 34:12636-45 (2014). IHC-FoFr ; Rat . PubMed: 25232103
- Semerdjieva S et al. Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors. Mol Cell Biol 33:1442-55 (2013). PubMed: 23382070