Sucrose Assay Kit (ab83387)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.2 µM
Overview
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Product name
Sucrose Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.2 µM -
Range
0 µM - 10 µM -
Assay time
1h 00m -
Product overview
Abcam's Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K626 Sucrose Colorimetric/Fluorometric Assay Kit. K626-100 is the same size as the 100 test size of ab83387.
Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer II 1 x 25ml Development Enzyme Mix II 1 vial Invertase 1 vial OxiRed Probe 1 x 200µl Sucrose Standard 1 x 100µl -
Research areas
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Relevance
Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose. -
Alternative names
- Saccharose
- Table sugar
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab83387 has been referenced in 1 publication.
- Maslova O et al. Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF). Bioresour Technol 272:1-9 (2019). PubMed: 30292911