Goat Anti-Human IgG (Fab')2 (HRP) (ab87422)
Key features and details
- Goat Anti-Human IgG (Fab')2 (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB
Overview
-
Product name
Goat Anti-Human IgG (Fab')2 (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
ab87422 is specific for the Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity. -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Other Immunogen Type corresponding to IgG. Human serum.
-
Conjugation
HRP
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.1% BSA, PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
This antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/1000 - 1/10000.
|
|
WB |
1/1000 - 1/10000.
|
Notes |
---|
ELISA
1/1000 - 1/10000. |
WB
1/1000 - 1/10000. |
Images
-
Characterisation of virus activity and functional expression of monoclonal antibodies.
(Panel D) IgG1 antibody expression, assessed by anti-IgG1 ELISA, in cellular supernatants of carcinoma cell lines infected for 24-96hrs with NG-135 at 1ppc.
Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab87422 has been referenced in 6 publications.
- Ehteshaminia Y et al. Enhancement of immunogenicity and neutralizing responses against SARS-CoV-2 spike protein using the Fc fusion fragment. Life Sci 320:121525 (2023). PubMed: 36841470
- Shi J et al. Effective vaccination strategy using SARS-CoV-2 spike cocktail against Omicron and other variants of concern. NPJ Vaccines 7:169 (2022). PubMed: 36535987
- Li Y et al. A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cells. Transl Oncol 21:101424 (2022). PubMed: 35477065
- Sasso E et al. Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies. MAbs 10:1060-1072 (2018). PubMed: 29995563
- Marino N et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). PubMed: 28542292
- Andrabi SA et al. Poly(ADP-ribose) polymerase-dependent energy depletion occurs through inhibition of glycolysis. Proc Natl Acad Sci U S A 111:10209-14 (2014). PubMed: 24987120