Anti-NPRL2 antibody (ab88691)
Key features and details
- Mouse polyclonal to NPRL2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NPRL2 antibody
See all NPRL2 primary antibodies -
Description
Mouse polyclonal to NPRL2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human NPRL2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: AAH21984.1 -
Positive control
- WB: HeLa cell lysate; NPRL2 transfected 293T cell lysate ICc/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs. -
Tissue specificity
Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested. -
Sequence similarities
Belongs to the NPR2 family. - Information by UniProt
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Database links
- Entrez Gene: 10641 Human
- Omim: 607072 Human
- SwissProt: Q8WTW4 Human
- Unigene: 437083 Human
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Alternative names
- 2810446G01Rik antibody
- G21 protein antibody
- Gene 21 protein antibody
see all
Images
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Anti-NPRL2 antibody (ab88691) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 44 kDa
Observed band size: 44 kDa -
All lanes : Anti-NPRL2 antibody (ab88691) at 1 µg/ml
Lane 1 : NPRL2 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 44 kDa
Observed band size: ~37 kDa why is the actual band size different from the predicted? -
ab88691, at 10 µg/ml, staining NPRL2 in HeLa cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab88691 has been referenced in 2 publications.
- Liu S & Liu B Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway. Med Sci Monit 24:1424-1433 (2018). PubMed: 29519997
- Liu AY et al. Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer. Cancer Biol Ther 15:489-95 (2014). PubMed: 24521741