Native human Plasmin protein (Active) (ab90928)
Key features and details
- Expression system: Native
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Native human Plasmin protein (Active)
See all Plasmin proteins and peptides -
Biological activity
15 units/mg protein
One unit is defined as the amount of enzyme that hydrolyzes one µmole of tosyl-Gly-Pro-Lys-pNA per minute at 25°C, pH 7.8. Note: One unit = 1.25 CU.
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Purity
>= 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
81 kDa
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab90928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Extinction Coeffcient (E) 0.1% at 280nm 1cm pathway =1.71
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionWe recommend that this product be resuspended in de-ionized H2O to the original volume.
General Info
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Alternative names
- Plasmin light chain B
- Plasminogen
- PLG
- PLMN_HUMAN
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Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
Tissue specificity
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
Involvement in disease
Plasminogen deficiency -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Domain
Kringle domains mediate interaction with CSPG4. -
Post-translational
modificationsN-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
Cellular localization
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
Images
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4-12% Bis-Tris NuPAGE gel.
Lane 1: Plasmin - 5 μg (reduced/ heated)
Lane 2: Plasmin - 10 μg (reduced/ heated)
Lane 3: Plasmin - 20 μg (reduced/ heated)
Lane 4: LMW Standard
Lane 5: Plasmin - 5 μg (non-reduced/ no heat)
Lane 6: Plasmin - 10 μg (non-reduced/ no heat)
Lane 7: Plasmin - 20 μg (non-reduced/ no heat)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab90928 has been referenced in 2 publications.
- Strittmatter T et al. Programmable DARPin-based receptors for the detection of thrombotic markers. Nat Chem Biol 18:1125-1134 (2022). PubMed: 35941237
- Nishimura M et al. Extracellular cleavage of collagen XVII is essential for correct cutaneous basement membrane formation. Hum Mol Genet 25:328-39 (2016). PubMed: 26604146