Recombinant Anti-Plexin A1 antibody [EPR3449] (ab92346)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3449] to Plexin A1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Plexin A1 antibody [EPR3449]
See all Plexin A1 primary antibodies -
Description
Rabbit monoclonal [EPR3449] to Plexin A1 -
Host species
Rabbit -
Specificity
ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally. -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Plexin A1 aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: KS62, HUVEC and HepG2 whole cell lysate (ab7900); fetal brain tissue lysate Flow Cyt (intra): K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
This antibody is not purified. It is provided in cell supernatant and storage buffer. -
Clonality
Monoclonal -
Clone number
EPR3449 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000 - 1/2000. Predicted molecular weight: 211 kDa.
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Notes |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Predicted molecular weight: 211 kDa. |
Target
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Relevance
Function: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 5361 Human
- Entrez Gene: 18844 Mouse
- NCBI: NP_115618 Human
- Omim: 601055 Human
- SwissProt: Q9UIW2 Human
- SwissProt: P70206 Mouse
- Unigene: 432329 Human
- Unigene: 3789 Mouse
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Alternative names
- NOV antibody
- NOV/plexin A1 protein antibody
- NOVP antibody
see all
Images
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All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 211 kDa -
Intracellular Flow Cytometry analysis of permeabilized K562 cells labeling Plexin A1 with ab92346 at 1/50 (red) campared to a rabbit IgG (negative control) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92346 has been referenced in 1 publication.
- Wu JH et al. Semaphorin-3A protects against neointimal hyperplasia after vascular injury. EBioMedicine 39:95-108 (2019). PubMed: 30579864