Recombinant Anti-S100A9 antibody [EPR3556] (ab92468)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3556] to S100A9
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-S100A9 antibody [EPR3556]
See all S100A9 primary antibodies -
Description
Rabbit monoclonal [EPR3556] to S100A9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC/IF,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human tonsil lysate and peripheral blood leukocyte cell lysate IHC-P: Human spleen tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3556 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Notes |
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WB: 1/1000 - 1/10,000. Predicted molecular weight: 13 kDa.
Is unsuitable for ICC/IF or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
Tissue specificity
Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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Database links
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P06702 Human
- Unigene: 112405 Human
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Alternative names
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab92468 has been referenced in 5 publications.
- Basuli D et al. Kinin B1 Receptor Mediates Renal Injury and Remodeling in Hypertension. Front Med (Lausanne) 8:780834 (2021). PubMed: 35118089
- Rong X et al. Antler stem cells as a novel stem cell source for reducing liver fibrosis. Cell Tissue Res 379:195-206 (2020). PubMed: 31428875
- Guo M et al. Metformin alleviates endometrial hyperplasia through the UCA1/miR-144/TGF-ß1/AKT signaling pathway. Int J Mol Med 45:623-633 (2020). PubMed: 31894313
- Wei J et al. Perinatal exposure to bisphenol A exacerbates nonalcoholic steatohepatitis-like phenotype in male rat offspring fed on a high-fat diet. J Endocrinol 222:313-25 (2014). PubMed: 25112833
- Junker H et al. Stage-Related Alterations in Renal Cell Carcinoma - Comprehensive Quantitative Analysis by 2D-DIGE and Protein Network Analysis. PLoS One 6:e21867 (2011). WB, IHC-P ; Human . PubMed: 21760917