Anti-Vav proteins antibody (ab92890)
Key features and details
- Rabbit polyclonal to Vav proteins
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Vav proteins antibody
See all Vav proteins primary antibodies -
Description
Rabbit polyclonal to Vav proteins -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to amino acids 172-176 of Human Vav proteins
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Positive control
- Jurkat or THP1 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 98 kDa.
Predicted molecular weight: 98 kDa (VAV1 & VAV3). 101 kDa (VAV2). |
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IP |
Use at an assay dependent concentration.
2 µg. |
Notes |
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WB
1/1000. Predicted molecular weight: 98 kDa. Predicted molecular weight: 98 kDa (VAV1 & VAV3). 101 kDa (VAV2). |
IP
Use at an assay dependent concentration. 2 µg. |
Target
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Relevance
The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response. -
Cellular localization
Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3 -
Database links
- Entrez Gene: 10451 Human
- Entrez Gene: 7409 Human
- Entrez Gene: 7410 Human
- Omim: 164875 Human
- Omim: 600428 Human
- Omim: 605541 Human
- SwissProt: P15498 Human
- SwissProt: P52735 Human
see all -
Alternative names
- 2810040F13Rik antibody
- A530094I06Rik antibody
- AA986410 antibody
see all
Images
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Immunoprecipitation of VAV proteins from 500µg of Jurkat (Lane 1) and THP1 (Lane 2) cell lysates, using 2µg of ab92890.
All lanes : Anti-Vav proteins antibody (ab92890) at 2 µg
Lane 1 : Jurkat cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 500 µg per lane. -
All lanes : Anti-Vav proteins antibody (ab92890) at 1/1000 dilution
Lane 1 : Jurkat cell lysate at 3 µg
Lane 2 : Jurkat cell lysate at 10 µg
Lane 3 : Jurkat cell lysate at 30 µg
Predicted band size: 98 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab92890 has not yet been referenced specifically in any publications.