Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab97077)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
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Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
Minimal
cross-reactivity
Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details -
Conjugation
Cy5 ®. Ex: 650nm, Em: 667nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/100 - 1/1000.
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IHC-P |
1/50 - 1/500.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/100 - 1/1000. |
IHC-P
1/50 - 1/500. |
Images
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Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells.
Rab7 was detected with an Rabbit anti-Rab7 antibody, followed by ab97077 at 1/500 dilution in ICC/IF analysis of HeLa cells.
(From Figure S1 of Humphries et al)
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Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3% PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1:100
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:100
Nuclei were counterstained with SYTO 16 -
ab97077 (1/200) detecting ab5176 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue); ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (34)
ab97077 has been referenced in 34 publications.
- Yoshida Y et al. Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue. iScience 25:104268 (2022). PubMed: 35521515
- Hayashi Y et al. Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity. iScience 25:104547 (2022). PubMed: 35754738
- Yoshida Y et al. Brown adipose tissue dysfunction promotes heart failure via a trimethylamine N-oxide-dependent mechanism. Sci Rep 12:14883 (2022). PubMed: 36050466
- Schmiderer L et al. Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import. Sci Rep 12:18687 (2022). PubMed: 36333335
- Bouchard G et al. Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge. Cancer Res 82:648-664 (2022). PubMed: 34853070