Recombinant
RabMAb

Recombinant Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free (ab232525)

Overview

  • Product name

    Anti-ABAT/GABA-T antibody [EPR4433] - BSA and Azide free
    See all ABAT/GABA-T primary antibodies
  • Description

    Rabbit monoclonal [EPR4433] to ABAT/GABA-T - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ABAT/GABA-T aa 1-100. The exact sequence is proprietary.
    Database link: P80404
    (Peptide available as ab202287)

  • Positive control

    • IHC-P: Human liver tissue.
  • General notes

    ab232525 is the carrier-free version of ab108249 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232525 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as ABAT.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

antigen retrieval is recommended

Target

  • Function

    Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
  • Tissue specificity

    Liver > pancreas > brain > kidney > heart > placenta.
  • Involvement in disease

    Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
  • Sequence similarities

    Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • (S) 3 amino 2 methylpropionate transaminase antibody
    • (S)-3-amino-2-methylpropionate transaminase antibody
    • 4 aminobutyrate aminotransferase antibody
    • 4 aminobutyrate aminotransferase, mitochondrial antibody
    • 4-aminobutyrate aminotransferase antibody
    • ABAT antibody
    • FLJ17813 antibody
    • FLJ30272 antibody
    • GABA aminotransferase antibody
    • GABA AT antibody
    • GABA T antibody
    • GABA transaminase antibody
    • GABA transferase antibody
    • GABA-AT antibody
    • GABA-T antibody
    • GABAT antibody
    • GABT_HUMAN antibody
    • Gamma amino N butyrate transaminase antibody
    • Gamma-amino-N-butyrate transaminase antibody
    • hCG1984265 antibody
    • L AIBAT antibody
    • L-AIBAT antibody
    • LAIBAT antibody
    • mitochondrial antibody
    • NPD009 antibody
    see all

Images

  • ab108249 (purified) at 1:40 dilution (2µg) immunoprecipitating ABAT/GABA-T in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab108249 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108249 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

  • Immunohistocemical analysis of human liver tissue labeling ABAT/GABA-T using ab108249 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108249).

References

ab232525 has not yet been referenced specifically in any publications.

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