Overview

  • Product name

  • Description

    Rabbit polyclonal to ABCA1
  • Host species

    Rabbit
  • Specificity

    ab7360 has previously been shown to work well in ICC/IF on HeLa cells. However recent batches of this antibody, do not work in this application. We would recommend ab18180 for researchers wanting to detect ABCA1 in ICC/IF. For further information, please contact our Scientific Support Team.
  • Tested applications

    Suitable for: IP, ELISA, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human ABCA1. Synthetic peptide: The immunogen is generated from within residues 1100-1300 of Human ABCA1
    Database link: O95477

Applications

Our Abpromise guarantee covers the use of ab7360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

PMID: 14660648

ELISA Use at an assay dependent concentration.

PMID: 21829447

WB 1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.
IHC-P 1/500.
IHC-Fr 1/750. PubMed: 12547833

Target

  • Function

    cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
  • Tissue specificity

    Widely expressed, but most abundant in macrophages.
  • Involvement in disease

    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Domain

    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Post-translational
    modifications

    Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC 1 antibody
    • ABC Transporter 1 antibody
    • ABC-1 antibody
    • ABC1 antibody
    • ABCA 1 antibody
    • ABCA1 antibody
    • ABCA1_HUMAN antibody
    • ATP binding Cassette 1 antibody
    • ATP binding cassette sub family A ABC1 member 1 antibody
    • ATP binding cassette sub family A member 1 antibody
    • ATP binding Cassette Transporter 1 antibody
    • ATP-binding cassette 1 antibody
    • ATP-binding cassette sub-family A member 1 antibody
    • ATP-binding cassette transporter 1 antibody
    • CERP antibody
    • Cholesterol efflux regulatory protein antibody
    • FLJ14958 antibody
    • HDLDT1 antibody
    • Membrane bound antibody
    • MGC164864 antibody
    • MGC165011 antibody
    • TD antibody
    • TGD antibody
    see all

Images

  • All lanes : Anti-ABCA1 antibody (ab7360)

    Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
    Lane 2 : RAW 264.7 whole cell lysate untreated

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 220 kDa

  • ab7360, staining ABCA1 in human prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.

    The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separ
  • Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
    Lane 4: T09 uninduced lysate
    Lane 5: T09 induced lysate Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
    Lane 4: T09 uninduced lysate
    Lane 5: T09 induced lysate

References

This product has been referenced in:

  • Takeuchi S  et al. Elevated Membrane Cholesterol Disrupts Lysosomal Degradation to Induce ß-Amyloid Accumulation: The Potential Mechanism Underlying Augmentation of ß-Amyloid Pathology by Type 2 Diabetes Mellitus. Am J Pathol 189:391-404 (2019). Read more (PubMed: 30448407) »
  • Liu JY  et al. Protective effect of down-regulated microRNA-27a mediating high thoracic epidural block on myocardial ischemia-reperfusion injury in mice through regulating ABCA1 and NF-?B signaling pathway. Biomed Pharmacother 112:108606 (2019). Read more (PubMed: 30802823) »
See all 45 Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa and pluripotent cells)
Permeabilization
Yes - Triton X-100
Specification
HeLa and pluripotent cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 14 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (SCC61 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
SCC61 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 13 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (human astrocyte)
Loading amount
25 µg
Specification
human astrocyte
Treatment
treated with LXR ligand for 20 hrs
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 06 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, THP1, mouse BMDM, mouse tissue)
Loading amount
15 µg
Specification
HEK293, THP1, mouse BMDM, mouse tissue
Treatment
Transfected with ABCA1 expression plasmid, Treat with 1uM TO-1317 for 24hr
Gel Running Conditions
Reduced Denaturing
Blocking step
5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 26 2008

Application
Western blot
Sample
Mouse Tissue lysate - whole (macrophage cell line)
Loading amount
15 µg
Specification
macrophage cell line
Treatment
0.3mM dbcAMP 24 hr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5

Abcam user community

Verified customer

Submitted Sep 18 2006

Application
Western blot
Sample
Human Tissue lysate - other (placenta, arteries)
Loading amount
50 µg
Specification
placenta, arteries
Treatment
50U Benzonuclease for 1 h
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 7

Abcam user community

Verified customer

Submitted Aug 23 2006

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