Western blot abreview for Anti-ABCA1 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (SCC61 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
SCC61 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/500

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 260 kDa Non-specific: 160, 60, 50, 40 kDa

Additional data

Additional Notes
Lane 1: Veh control Lane 2: 22(R) OH Cholesterol Lane 3: DDA Lane 4: Standard

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Submitted Nov 13 2015

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