I've tried 2 different batches of this antibody. Both do not localise to the plasma membrane as expected in HeLa nor H7.s14 pluripotent stem cells. In my hands this antibody did not behave as described by Frechin et al. 2015.
Thank you for sharing this data with us. Previous batches of ab7360 have been shown to work in ICC/IF on HeLa cells. However, based on the data shown here, current batches of ab7360 do not work in ICC/IF on this cell line, under these experimental conditions. We would welcome feedback from other researchers using this antibody.
Abcam user community
Submitted Apr 14 2016
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