Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCA1 antibody

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa and pluripotent cells)
Permeabilization
Yes - Triton X-100
Specification
HeLa and pluripotent cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/5% serum
Concentration
2 µg/ml

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: Goat anti-Rabbit AlexaFluor
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
I've tried 2 different batches of this antibody. Both do not localise to the plasma membrane as expected in HeLa nor H7.s14 pluripotent stem cells. In my hands this antibody did not behave as described by Frechin et al. 2015.
Abcam response
Thank you for sharing this data with us. Previous batches of ab7360 have been shown to work in ICC/IF on HeLa cells. However, based on the data shown here, current batches of ab7360 do not work in ICC/IF on this cell line, under these experimental conditions. We would welcome feedback from other researchers using this antibody.

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Submitted Apr 14 2016

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