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  1. Link

    abca1-antibody-abh10-ab18180.pdf

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Neuroscience Cell Type Marker Glia marker Astrocyte marker
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Validated using a knockout cell line

Anti-ABCA1 antibody [AB.H10] (ab18180)

  • Datasheet
  • SDS
Reviews (18)Q&A (36)References (165)

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Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)
  • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
  • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)

Key features and details

  • Mouse monoclonal [AB.H10] to ABCA1
  • Suitable for: IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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View more associated products

Overview

  • Product name

    Anti-ABCA1 antibody [AB.H10]
    See all ABCA1 primary antibodies
  • Description

    Mouse monoclonal [AB.H10] to ABCA1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
    Database link: O95477

  • Positive control

    • testis, liver, and brain tissue (negative control: muscle tissue)
  • General notes

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    AB.H10
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human ABCA1 protein (ab125995)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt (3)
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (13)
1/200 - 1/500. Predicted molecular weight: 254 kDa.

Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.

Notes
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/200 - 1/500. Predicted molecular weight: 254 kDa.

Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.

Target

  • Function

    cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
  • Tissue specificity

    Widely expressed, but most abundant in macrophages.
  • Involvement in disease

    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Domain

    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Post-translational
    modifications

    Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession O95477 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 19 Human
    • Entrez Gene: 11303 Mouse
    • Omim: 600046 Human
    • SwissProt: O95477 Human
    • SwissProt: P41233 Mouse
    • Unigene: 429294 Human
    • Unigene: 277376 Mouse
    • Alternative names

      • ABC 1 antibody
      • ABC Transporter 1 antibody
      • ABC-1 antibody
      • ABC1 antibody
      • ABCA 1 antibody
      • ABCA1 antibody
      • ABCA1_HUMAN antibody
      • ATP binding Cassette 1 antibody
      • ATP binding cassette sub family A ABC1 member 1 antibody
      • ATP binding cassette sub family A member 1 antibody
      • ATP binding Cassette Transporter 1 antibody
      • ATP-binding cassette 1 antibody
      • ATP-binding cassette sub-family A member 1 antibody
      • ATP-binding cassette transporter 1 antibody
      • CERP antibody
      • Cholesterol efflux regulatory protein antibody
      • FLJ14958 antibody
      • HDLDT1 antibody
      • Membrane bound antibody
      • MGC164864 antibody
      • MGC165011 antibody
      • TD antibody
      • TGD antibody
      see all

    Images

    • Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.
    • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      All lanes : Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution

      Lanes 1 & 3 & 5 : Wild type mouse liver tissue lysate
      Lanes 2 & 4 & 6 : Knockout mouse liver tissue lysate

      Lysates/proteins at 100 µg per lane.

      Predicted band size: 254 kDa
      Observed band size: 254 kDa



      The lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.

    Protocols

    • Recommended protocol for IHC-P with ab18180

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (165)

    Publishing research using ab18180? Please let us know so that we can cite the reference in this datasheet.

    ab18180 has been referenced in 165 publications.

    • Pi S  et al. The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis. Autophagy 17:980-1000 (2021). PubMed: 32160082
    • Wanschel ACBA  et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction. Biomolecules 11:N/A (2021). PubMed: 33430172
    • Kim JS  et al. Melatonin activates ABCA1 via the BiP/NRF1 pathway to suppress high-cholesterol-induced apoptosis of mesenchymal stem cells. Stem Cell Res Ther 12:114 (2021). PubMed: 33546749
    • Luo J  et al. Ad- and AAV8-mediated ABCA1 gene therapy in a murine model with retinal ischemia/reperfusion injuries. Mol Ther Methods Clin Dev 20:551-558 (2021). PubMed: 33665225
    • Gnanaguru G  et al. Targeting of miR-33 ameliorates phenotypes linked to age-related macular degeneration. Mol Ther 29:2281-2293 (2021). PubMed: 33744470
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 54 Abreviews or Q&A

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Guinea pig Tissue lysate - other (Brain membrane)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Brain membrane
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 02 2022

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Cell lysate - whole cell (Ovine Thecal Cells)
    Gel Running Conditions
    Reduced Denaturing (10% PAG)
    Loading amount
    10 µg
    Specification
    Ovine Thecal Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Barbara Makela

    Verified customer

    Submitted Jun 22 2017

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Loading amount
    25 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 24 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCA1 antibody [AB.H10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary astrocyte culture)
    Permeabilization
    Yes - 0.1M PBS with 1% Triton X
    Specification
    Primary astrocyte culture
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 06 2016

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Synovial membrane)
    Gel Running Conditions
    Reduced Denaturing (8 %)
    Loading amount
    35 µg
    Specification
    Synovial membrane
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 13 2016

    Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - BD PERMEABILISATION SOLUTION
    Sample
    Mouse Cell (liver cells)
    Specification
    liver cells
    Gating Strategy
    CD24 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: single cell suspension
    Sample buffer: 0.1%PBS-BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 10 2013

    Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - BD Permeabilisation Solution
    Sample
    Rat Cell (Liver cells)
    Specification
    Liver cells
    Gating Strategy
    CD24 Positive
    Preparation
    Cell harvesting/tissue preparation method: Single cell suspension
    Sample buffer: 0.1% PBS-BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 05 2013

    Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - BD PERMEABILISATION SOLUTION
    Sample
    Human Cell (HEP G2)
    Specification
    HEP G2
    Gating Strategy
    CD24 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: single cell suspension
    Sample buffer: 0.1%PBS-BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. brahmi manali

    Verified customer

    Submitted Dec 04 2013

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (small intestine)
    Loading amount
    30 µg
    Specification
    small intestine
    Gel Running Conditions
    Reduced Denaturing (7)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Dongil Kim

    Verified customer

    Submitted Apr 23 2013

    Question

    Thank you for your kind replacement of the antibody! I was able to test it on human prostate cancer cell line PC3 lysed with RIPA buffer (last lane of the samples indicated by arrow) and my other prostate cancer cell lines prepared before. The membrane was first detected with your new antibody and then reprobed with FASN. As you can see from the images, your new antibody can only show a low molecular weight background band. FASN antibody clearly detects the target protein with several non-specific bands. The pictures in two different pages represents different exposure time. Therefore, I am afraid that your new antibody does not work either. If you can provide a positive control, I am willing to repeat experiment again. If your positive control works, it means that your antibodies work. I am happy to keep them and improve my sample preparation.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 08 2013

    Answer


    Yes, I can send you as a one time exception a positive control lysate (rat liver (ab29413), https://www.abcam.com/liver-rat-whole-cell-lysate-normal-tissue-ab29413.html). This has been tested with ab66217 and gave strong bands.

    Also I would suggest to check the gel after transfer as some of the high MW proteins might have not completely transfered onto the membrane. You can check this by staining the gel with Coomassie Blue. if you see bands in the gel at 200 kDa and higher, you might want to adjust your transfer conditions so that all high MW protein are being transfered.

    Also, avoid boiling of your samples before loading them onto the gel. Warming your sample to 50-60 degree Celsius is sufficient for denaturation in loading buffer.

    Again, FASN is a cytoplasmic protein and such is much easier to work with than ABCA1 which is very hydrophobic, glycosylated and of high MW.

    This article and link might be of help as well: http://www.sciencedirect.com/science/article/pii/S0003269708006829
    http://www.scientistsolutions.com/t14685-membrane+protein+western+blot.html

    Read More

    Abcam Scientific Support

    Answered on Jan 08 2013

    1-10 of 54 Abreviews or Q&A

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