Anti-ABCA1 antibody [AB.H10] (ab18180)
![Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)](https://www.abcam.com/ps/products/18/ab18180/Images/ab18180-26823-anti-abca1-antibody-abh10-flow-cytometry.jpg "Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)")
Key features and details
- Mouse monoclonal [AB.H10] to ABCA1
- Suitable for: IHC-P, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-ABCA1 antibody [AB.H10]
See all ABCA1 primary antibodies -
Description
Mouse monoclonal [AB.H10] to ABCA1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
Database link: O95477 -
Positive control
- testis, liver, and brain tissue (negative control: muscle tissue)
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General notes
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A purified from TCS -
Clonality
Monoclonal -
Clone number
AB.H10 -
Isotype
IgG1 -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| Flow Cyt | (3) |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| WB | (12) |
1/200 - 1/500. Predicted molecular weight: 254 kDa.
Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate. |
| Notes |
|---|
|
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
1/200 - 1/500. Predicted molecular weight: 254 kDa. Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate. |
Target
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Function
cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. -
Tissue specificity
Widely expressed, but most abundant in macrophages. -
Involvement in disease
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. -
Domain
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
Post-translational
modificationsPhosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 19 Human
- Entrez Gene: 11303 Mouse
- Omim: 600046 Human
- SwissProt: O95477 Human
- SwissProt: P41233 Mouse
- Unigene: 429294 Human
- Unigene: 277376 Mouse
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Alternative names
- ABC 1 antibody
- ABC Transporter 1 antibody
- ABC-1 antibody
see all
Images
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Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
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![Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)](https://www.abcam.com/ps/products/18/ab18180/Images/ab18180-26825-anti-abca1-antibody-abh10-western-blot.jpg)
Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control. -
![Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)](https://www.abcam.com/ps/products/18/ab18180/Images/ab18180-242310-anti-abca1-antibody-abh10-western-blot.jpg)
Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)All lanes : Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution
Lanes 1 & 3 & 5 : Wild type mouse liver tissue lysate
Lanes 2 & 4 & 6 : Knockout mouse liver tissue lysate
Lysates/proteins at 100 µg per lane.
Predicted band size: 254 kDa
Observed band size: 254 kDaThe lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)](https://www.abcam.com/ps/products/18/ab18180/Images/ab18180-26824-anti-abca1-antibody-abh10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.
Datasheets and documents
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SDS download
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Datasheet download
References (165)
ab18180 has been referenced in 165 publications.
- Pi S et al. The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis. Autophagy 17:980-1000 (2021). PubMed: 32160082
- Wanschel ACBA et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction. Biomolecules 11:N/A (2021). PubMed: 33430172
- Kim JS et al. Melatonin activates ABCA1 via the BiP/NRF1 pathway to suppress high-cholesterol-induced apoptosis of mesenchymal stem cells. Stem Cell Res Ther 12:114 (2021). PubMed: 33546749
- Luo J et al. Ad- and AAV8-mediated ABCA1 gene therapy in a murine model with retinal ischemia/reperfusion injuries. Mol Ther Methods Clin Dev 20:551-558 (2021). PubMed: 33665225
- Gnanaguru G et al. Targeting of miR-33 ameliorates phenotypes linked to age-related macular degeneration. Mol Ther 29:2281-2293 (2021). PubMed: 33744470