Recombinant
RabMAb

Recombinant Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

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Overview

  • Product name

    Anti-ABCA1 (phospho S2054) antibody [EPR2485]
    See all ABCA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2485] to ABCA1 (phospho S2054)

  • Host species

    Rabbit

  • Specificity

    ab125064 only detects ABCA1 phosphorylated at serine 2054. Cross reactivity with mouse was determined by sequence analysis only.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCA1 aa 2050-2150 (phospho S2054). The exact sequence is proprietary.
    Database link: O95477

  • Positive control

    • WB: Okadaic Acid and Calyculin A treated SH-SY5Y cell lysates. ICC/IF: Okadaic Acid and Calyculin A treated SH-SY5Y cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab125064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 240 kDa (predicted molecular weight: 254 kDa).
ICC/IF 1/500 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.

    • Tissue specificity

      Widely expressed, but most abundant in macrophages.

    • Involvement in disease

      Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
      Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.

    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCA family.
      Contains 2 ABC transporter domains.

    • Domain

      Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.

    • Post-translational
      modifications

      Phosphorylation on Ser-2054 regulates phospholipid efflux.
      Palmitoylation by DHHC8 is essential for membrane localization.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ABC 1 antibody
        • ABC Transporter 1 antibody
        • ABC-1 antibody
        • ABC1 antibody
        • ABCA 1 antibody
        • ABCA1 antibody
        • ABCA1_HUMAN antibody
        • ATP binding Cassette 1 antibody
        • ATP binding cassette sub family A ABC1 member 1 antibody
        • ATP binding cassette sub family A member 1 antibody
        • ATP binding Cassette Transporter 1 antibody
        • ATP-binding cassette 1 antibody
        • ATP-binding cassette sub-family A member 1 antibody
        • ATP-binding cassette transporter 1 antibody
        • CERP antibody
        • Cholesterol efflux regulatory protein antibody
        • FLJ14958 antibody
        • HDLDT1 antibody
        • Membrane bound antibody
        • MGC164864 antibody
        • MGC165011 antibody
        • TD antibody
        • TGD antibody
        see all

      Images

      • Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
        Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
        All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/200000 dilution

        Lane 1 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 (pS2054) peptide
        Lane 2 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 non-phospho peptide

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 254 kDa
        Observed band size: 240 kDa
        why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking and dilution buffer: 5% BSA/TBST.

      • Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
        Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
        All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/50000 dilution

        Lane 1 : SH-SY5Y cell lysate, untreated
        Lane 2 : SH-SY5Y cell lysate, Okadaic Acid and Calyculin A treated

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 254 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
        Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
        ab125064, at a 1/500 dilution, staining ABCA1 (green) in Okadaic Acid and Calyculin A treated SH-SY5Y cells by immunofluorescence. DNA is marked in blue.

      References

      ab125064 has not yet been referenced specifically in any publications.

      Publishing research using ab125064? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-ABCA1 (phospho S2054) antibody [EPR2485]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (macrophages RAW264.7)
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Loading amount
      22 µg
      Treatment
      4µM TO-901317, 18h (both lanes), 100ng/ml LPS 10min
      Specification
      macrophages RAW264.7
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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      Submitted Jun 08 2018

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