Recombinant
Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-ABCA1 (phospho S2054) antibody [EPR2485]
See all ABCA1 primary antibodies -
DescriptionRabbit monoclonal [EPR2485] to ABCA1 (phospho S2054)
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Host speciesRabbit
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Specificityab125064 only detects ABCA1 phosphorylated at serine 2054. Cross reactivity with mouse was determined by sequence analysis only.
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCA1 aa 2050-2150 (phospho S2054). The exact sequence is proprietary.
Database link: O95477 -
Positive control
- WB: Okadaic Acid and Calyculin A treated SH-SY5Y cell lysates. ICC/IF: Okadaic Acid and Calyculin A treated SH-SY5Y cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR2485
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IsotypeIgG
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Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Applications
Our Abpromise guarantee covers the use of ab125064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000 - 1/200000. Detects a band of approximately 240 kDa (predicted molecular weight: 254 kDa). | |
| ICC/IF | 1/500 - 1/1000. |
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
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FunctioncAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
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Tissue specificityWidely expressed, but most abundant in macrophages.
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Involvement in diseaseDefects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux. -
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. -
DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
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Post-translational
modificationsPhosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 19 Human
- Entrez Gene: 11303 Mouse
- Omim: 600046 Human
- SwissProt: O95477 Human
- SwissProt: P41233 Mouse
- Unigene: 429294 Human
- Unigene: 277376 Mouse
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Alternative names
- ABC 1 antibody
- ABC Transporter 1 antibody
- ABC-1 antibody
see all
Images
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![Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)](https://www.abcam.com/ps/products/125/ab125064/Images/ab125064-255476-ab125064PB.jpg)
Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/200000 dilution
Lane 1 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 (pS2054) peptide
Lane 2 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 non-phospho peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 254 kDa
Observed band size: 240 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutesBlocking and dilution buffer: 5% BSA/TBST.
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![Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)](https://www.abcam.com/ps/datasheet/images/125/ab125064/ABCA1-Primary-antibodies-ab125064-2.jpg)
Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/50000 dilution
Lane 1 : SH-SY5Y cell lysate, untreated
Lane 2 : SH-SY5Y cell lysate, Okadaic Acid and Calyculin A treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 254 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)](https://www.abcam.com/ps/datasheet/images/125/ab125064/ABCA1-Primary-antibodies-ab125064-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)ab125064, at a 1/500 dilution, staining ABCA1 (green) in Okadaic Acid and Calyculin A treated SH-SY5Y cells by immunofluorescence. DNA is marked in blue.
References
ab125064 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"