Recombinant
RabMAb

Recombinant Anti-ABCA1 (phospho S2054) antibody [EPR2485] - BSA and Azide free (ab248108)

Overview

  • Product name

    Anti-ABCA1 (phospho S2054) antibody [EPR2485] - BSA and Azide free
    See all ABCA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2485] to ABCA1 (phospho S2054) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects ABCA1 phosphorylated at serine 2054. Cross reactivity with mouse was determined by sequence analysis only.

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ABCA1 aa 2050-2150 (phospho S2054). The exact sequence is proprietary.
    Database link: O95477

  • General notes

    Ab248108 is the carrier-free version of ab125064. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248108 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab248108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 240 kDa (predicted molecular weight: 254 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
    • Tissue specificity

      Widely expressed, but most abundant in macrophages.
    • Involvement in disease

      Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
      Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCA family.
      Contains 2 ABC transporter domains.
    • Domain

      Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
    • Post-translational
      modifications

      Phosphorylation on Ser-2054 regulates phospholipid efflux.
      Palmitoylation by DHHC8 is essential for membrane localization.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ABC 1 antibody
      • ABC Transporter 1 antibody
      • ABC-1 antibody
      • ABC1 antibody
      • ABCA 1 antibody
      • ABCA1 antibody
      • ABCA1_HUMAN antibody
      • ATP binding Cassette 1 antibody
      • ATP binding cassette sub family A ABC1 member 1 antibody
      • ATP binding cassette sub family A member 1 antibody
      • ATP binding Cassette Transporter 1 antibody
      • ATP-binding cassette 1 antibody
      • ATP-binding cassette sub-family A member 1 antibody
      • ATP-binding cassette transporter 1 antibody
      • CERP antibody
      • Cholesterol efflux regulatory protein antibody
      • FLJ14958 antibody
      • HDLDT1 antibody
      • Membrane bound antibody
      • MGC164864 antibody
      • MGC165011 antibody
      • TD antibody
      • TGD antibody
      see all

    References

    ab248108 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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