Anti-ABCA12 antibody (ab98976)


  • Product name
    Anti-ABCA12 antibody
  • Description
    Rabbit polyclonal to ABCA12
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1979-2028 (TTIFKMLTGD IIPSSGNILI RNKTGSLGHV DSHSSLVGYC PQEDALDDLV) of Human ABCA12 (NP_056472; UniProt ID: Q86UK0 isoform 2).

  • Positive control
    • Human fetal stomach lysate



Our Abpromise guarantee covers the use of ab98976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 257 kDa.Can be blocked with ABCA12 peptide (ab127886). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Probable transporter involved in lipid homeostasis.
  • Tissue specificity
    Mainly expressed in the stomach, placenta, testis and fetal brain.
  • Involvement in disease
    Defects in ABCA12 are the cause of ichthyosis harlequin (HI) [MIM:242500]; also known as harlequin fetus. HI is a very severe skin disorder in which the neonate is born with a thick covering of armor-like scales. The skin dries out to form hard diamond-shaped plaques separated by fissures, resembling 'armor plating'. The normal facial features are severely affected, with distortion of the lips (eclabion), eyelids (ectropion), ears, and nostrils. Affected babies are often born prematurely and rarely survive the perinatal period.
    Defects in ABCA12 are the cause of ichthyosis lamellar type 2 (LI2) [MIM:601277]; also known as ichthyosis congenita IIB (ICR2B). LI is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. It is one the most severe forms of ichthyoses apparent at birth and persisting throughout life. LI patients are born encased in a tight, shiny, translucent covering called collodion membrane. Over the first weeks of life, the collodion membrane is gradually replaced by generalized large, dark brown, plate-like scales with minimal to no erythroderma. Tautness of facial skin commonly results in ectropion, eclabium and scarring alopecia of the scalp. Common complications are severe heat intolerance and recurrent ear infections.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Domain
    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC transporter A family member 12 antibody
    • ABC transporter ABCA.12 antibody
    • ABC12 antibody
    • ABCA12 antibody
    • ABCAC_HUMAN antibody
    • AtABCA12 antibody
    • ATH16 antibody
    • ATP binding cassette 12 antibody
    • ATP binding cassette sub family A (ABC1) member 12 antibody
    • ATP binding cassette sub family A member 12 antibody
    • ATP binding cassette transporter 12 antibody
    • ATP-binding cassette 12 antibody
    • ATP-binding cassette sub-family A member 12 antibody
    • ATP-binding cassette transporter 12 antibody
    • Ichthyosis congenita II lamellar ichthyosis B antibody
    • ICR2B antibody
    • LI2 antibody
    • Putative ABC2 homolog 16 antibody
    see all


  • Anti-ABCA12 antibody (ab98976) at 1 µg/ml + Human fetal stomach lysate at 10 µg

    Predicted band size: 257 kDa

    Gel concentration: 6-18%


ab98976 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (human primary fibroblast)
Loading amount
30 µg
human primary fibroblast
Gel Running Conditions
Reduced Denaturing (7% acrylamide gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Jian Yuan-Ren

Verified customer

Submitted Apr 12 2012

Thank you very much for your reply.

I understand the customer's frustration, however I can not offer a free of charge replacement in this case as the antibody was working as stated on the datasheet.

If the customercould submit an Ab...

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Thank you for this update!

I am very happy to hear that the suggestions seemed to have resolved the problem. Unfortunately, there was no image attached to the email. Can you please send it again?

Thank you very much!

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your enquiry. I can confirm that as the datasheet indicates ab98976 has only been tested and characterized for Western blot application. Currently, we do not have any data regarding IHC, though it does not necessary mean that ab98976 i...

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Thank you very much for your kind reply and the image.

Indeed, the results look very good to me. If I understood correctly, the 3rd band is where the customer used the sample with the mutated ABCA12.

The second band all on top could...

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