Overview

  • Product name
    Anti-ABCA2 antibody
    See all ABCA2 primary antibodies
  • Description
    Rabbit polyclonal to ABCA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Dog, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 2300 to the C-terminus of Human ABCA2.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in Human Hippocampus formalin fixed paraffin embedded tissue section within immunohistochemistry as well as SKNSH cells within immunofluorescence. This antibody gave a positive signal in methanol fixed SH-SY5Y cells within Flow Cytometry.

Properties

Applications

Our Abpromise guarantee covers the use of ab65589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Probable transporter, its natural substrate has not been found yet. May have a role in macrophage lipid metabolism and neural development.
  • Tissue specificity
    Highy expressed in the brain, whereas lower levels of expression is observed in kidney and liver.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Endosome. Membrane. Lysosome. Membrane. Forms discrete, punctate intracellular vesicles.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ABC2 antibody
    • Abca2 antibody
    • ABCA2_HUMAN antibody
    • ATP binding cassette 2 antibody
    • ATP binding cassette sub family A member 2 antibody
    • ATP binding cassette transporter 2 antibody
    • ATP binding cassette, sub family A (ABC1), member 2 antibody
    • ATP binding cassette, sub family A, member 2 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 2 antibody
    • ATP-binding cassette 2 antibody
    • ATP-binding cassette sub-family A member 2 antibody
    • ATP-binding cassette transporter 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA2 antibody (ab65589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA2 antibody (ab65589)
    IHC image of ABCA2 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab704917, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCA2 antibody (ab65589)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCA2 antibody (ab65589)
    ICC/IF image of ab65589 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65589, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Flow Cytometry - Anti-ABCA2 antibody (ab65589)
    Flow Cytometry - Anti-ABCA2 antibody (ab65589)
    Overlay histogram showing SH-SY5Y cells stained with ab65589 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65589, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/1000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

ab65589 has not yet been referenced specifically in any publications.

Publishing research using ab65589? Please let us know so that we can cite the reference in this datasheet.

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