Anti-ABCA4 antibody [3F4] (ab77285)
Key features and details
- Mouse monoclonal [3F4] to ABCA4
- Suitable for: IHC-FoFr, WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human, Xenopus laevis
- Isotype: IgG
Overview
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Product name
Anti-ABCA4 antibody [3F4]
See all ABCA4 primary antibodies -
Description
Mouse monoclonal [3F4] to ABCA4 -
Host species
Mouse -
Tested applications
Suitable for: IHC-FoFr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Xenopus laevis -
Immunogen
Full length native protein (purified) corresponding to Cow ABCA4. 220 kDa
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Epitope
Epitope has been mapped to aa 2252 – 2262 bovine ABCA4 protein, see Pubmed 9092582 -
Positive control
- Adult mouse retina tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab77285 is protein G purified from culture supernatant. -
Clonality
Monoclonal -
Clone number
3F4 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20436469
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WB |
1/1000. Predicted molecular weight: 257 kDa.
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IHC-P |
1/100.
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Notes |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 20436469 |
WB
1/1000. Predicted molecular weight: 257 kDa. |
IHC-P
1/100. |
Target
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Function
In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery. -
Tissue specificity
Retinal-specific. Seems to be exclusively found in the rims of rod photoreceptor cells. -
Involvement in disease
Defects in ABCA4 are the cause of Stargardt disease type 1 (STGD1) [MIM:248200]. STGD is one of the most frequent causes of macular degeneration in childhood. It is characterized by macular dystrophy with juvenile-onset, rapidly progressive course, alterations of the peripheral retina, and subretinal deposition of lipofuscin-like material. STGD1 inheritance is autosomal recessive.
Defects in ABCA4 are the cause of fundus flavimaculatus (FFM) [MIM:248200]. FFM is an autosomal recessive retinal disorder very similar to Stargardt disease. In contrast to Stargardt disease, FFM is characterized by later onset and slowly progressive course.
Defects in ABCA4 may be a cause of age-related macular degeneration type 2 (ARMD2) [MIM:153800]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in ABCA4 are the cause of cone-rod dystrophy type 3 (CORD3) [MIM:604116]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
Defects in ABCA4 are the cause of retinitis pigmentosa type 19 (RP19) [MIM:601718]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP19 is characterized by choroidal atrophy. Inheritance is autosomal recessive. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. -
Cellular localization
Membrane. Localized to outer segment disk edges of rods and cones, with around one million copies/photoreceptor. - Information by UniProt
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Database links
- Entrez Gene: 24 Human
- Entrez Gene: 11304 Mouse
- Entrez Gene: 310836 Rat
- Omim: 601691 Human
- SwissProt: P78363 Human
- SwissProt: O35600 Mouse
- Unigene: 416707 Human
- Unigene: 3918 Mouse
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Alternative names
- ABC 10 antibody
- ABC A4 antibody
- ABC transporter, retinal-specific antibody
see all
Images
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ab77285, at a 1/100 dilution, staining ABCA4 in formalin fixed, paraffin embedded adult mouse retina tissue by Immunohistochemisty.
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All lanes : Anti-ABCA4 antibody [3F4] (ab77285) at 1/500 dilution
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : Rat Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 257 kDa
Observed band size: 257 kDa
Additional bands at: 142 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab77285 has been referenced in 3 publications.
- Partida GJ et al. Thy1 associates with the cation channel subunit HCN4 in adult rat retina. Invest Ophthalmol Vis Sci 53:1696-703 (2012). WB ; Rat . PubMed: 22281825
- Beaty TH et al. A genome-wide association study of cleft lip with and without cleft palate identifies risk variants near MAFB and ABCA4. Nat Genet 42:525-9 (2010). IHC-FoFr ; Mouse . PubMed: 20436469
- Illing M et al. The 220-kDa rim protein of retinal rod outer segments is a member of the ABC transporter superfamily. J Biol Chem 272:10303-10 (1997). PubMed: 9092582