• Product name
  • Description
    Rabbit polyclonal to ABCA4
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 2250 to the C-terminus of Human ABCA4.

    (Peptide available as ab87350.)

  • Positive control
    • This antibody gave a positive signal in Human and Mouse Retina Tissue lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab72955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 238 kDa (predicted molecular weight: 256 kDa).


  • Function
    In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.
  • Tissue specificity
    Retinal-specific. Seems to be exclusively found in the rims of rod photoreceptor cells.
  • Involvement in disease
    Defects in ABCA4 are the cause of Stargardt disease type 1 (STGD1) [MIM:248200]. STGD is one of the most frequent causes of macular degeneration in childhood. It is characterized by macular dystrophy with juvenile-onset, rapidly progressive course, alterations of the peripheral retina, and subretinal deposition of lipofuscin-like material. STGD1 inheritance is autosomal recessive.
    Defects in ABCA4 are the cause of fundus flavimaculatus (FFM) [MIM:248200]. FFM is an autosomal recessive retinal disorder very similar to Stargardt disease. In contrast to Stargardt disease, FFM is characterized by later onset and slowly progressive course.
    Defects in ABCA4 may be a cause of age-related macular degeneration type 2 (ARMD2) [MIM:153800]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in ABCA4 are the cause of cone-rod dystrophy type 3 (CORD3) [MIM:604116]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
    Defects in ABCA4 are the cause of retinitis pigmentosa type 19 (RP19) [MIM:601718]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP19 is characterized by choroidal atrophy. Inheritance is autosomal recessive.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Membrane. Localized to outer segment disk edges of rods and cones, with around one million copies/photoreceptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 10 antibody
    • ABC A4 antibody
    • ABC transporter, retinal-specific antibody
    • ABC10 antibody
    • ABCA 4 antibody
    • abcA4 antibody
    • ABCA4_HUMAN antibody
    • ABCR antibody
    • ARMD 2 antibody
    • ARMD2 antibody
    • ATP binding cassette 10 antibody
    • ATP binding cassette sub family A member 4 antibody
    • ATP binding cassette sub family A member4 antibody
    • ATP binding cassette transporter antibody
    • ATP binding cassette transporter retinal specific antibody
    • ATP binding cassette, sub family A (ABC1), member 4 antibody
    • ATP binding cassette, sub family A (ABC1), member4 antibody
    • ATP binding cassette10 antibody
    • ATP binding transporter, retina specific antibody
    • ATP-binding cassette sub-family A member 4 antibody
    • CORD 3 antibody
    • CORD3 antibody
    • DKFZp781N1972 antibody
    • FFM antibody
    • FLJ17534 antibody
    • Photoreceptor rim protein antibody
    • Retina specific ABC transporter antibody
    • Retinal specific ATP binding cassette transporter antibody
    • Retinal-specific ATP-binding cassette transporter antibody
    • RIM ABC transporter antibody
    • RIM protein antibody
    • RmP antibody
    • RP 19 antibody
    • RP19 antibody
    • Stargardt disease protein antibody
    • STGD antibody
    • STGD1 antibody
    see all


  • All lanes : Anti-ABCA4 antibody (ab72955) at 1 µg/ml

    Lane 1 : Eye (Human) - adult normal Tissue Lysate
    Lane 2 : Retina (Mouse) Tissue Lysate
    Lane 3 : Retina (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 256 kDa
    Observed band size: 235 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 31 kDa, 41 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab72955 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.


This product has been referenced in:
  • McClements ME  et al. A fragmented adeno-associated viral dual vector strategy for treatment of diseases caused by mutations in large genes leads to expression of hybrid transcripts. J Genet Syndr Gene Ther 7:N/A (2016). Read more (PubMed: 28239514) »

See 1 Publication for this product

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