Overview

  • Product name
  • Description
    Rabbit polyclonal to ABCB11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within internal amino acids 474-745 of Human ABCB11 (O95342).

  • Positive control
    • Jurkat whole cell lysate, AGS xenograft, A431 cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 146 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
  • Tissue specificity
    Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
  • Involvement in disease
    Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
    Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Domain
    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC member 16, MDR/TAP subfamily antibody
    • ABC16 antibody
    • ABCB1 antibody
    • Abcb11 antibody
    • ABCBB_HUMAN antibody
    • ATP binding cassette sub family B (MDR/TAP) member 11 antibody
    • ATP binding cassette sub family B member 11 antibody
    • ATP-binding cassette sub-family B member 11 antibody
    • Bile salt export pump antibody
    • BRIC2 antibody
    • Bsep antibody
    • PFIC 2 antibody
    • PFIC2 antibody
    • PGY4 antibody
    • progressive familial intrahepatic cholestasis 2 antibody
    • Sister of P glycoprotein antibody
    • Spgp antibody
    see all

Images

  • Anti-ABCB11 antibody (ab155421) at 1/1000 dilution + Jurkat whole cell
    lysate at 30 µg

    Predicted band size: 146 kDa



    5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling ABCB11 with ab155421 at 1/500. Image at the right costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded AGS xenograft labeling ABCB11 with ab155421 antibody at 1/100.

References

This product has been referenced in:
  • Mather Q  et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »
  • Zhou JJ  et al. Knockdown of long non-coding RNA HOTAIR sensitizes hepatocellular carcinoma cell to cisplatin by suppressing the STAT3/ABCB1 signaling pathway. Oncol Lett 14:7986-7992 (2017). Read more (PubMed: 29250186) »
See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (hepatocellular carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0, 20 min, 100C
Permeabilization
No
Specification
hepatocellular carcinoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

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Verified customer

Submitted Dec 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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