Key features and details
- Rabbit polyclonal to ABCB11/BSEP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ABCB11/BSEP antibody
See all ABCB11/BSEP primary antibodies
DescriptionRabbit polyclonal to ABCB11/BSEP
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ABCB11/BSEP aa 474-745 (internal sequence).
Database link: O95342
- Jurkat whole cell lysate (ab7899), AGS xenograft, A431 cells.
General notesKeep as concentrated solution.
This product was previously labelled as ABCB11
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab155421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 146 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
Tissue specificityExpressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
Involvement in diseaseDefects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
- Information by UniProt
- ABC member 16, MDR/TAP subfamily antibody
- ABC16 antibody
- ABCB1 antibody
Immunohistochemical analysis of paraffin embedded AGS xenograft labeling ABCB11/BSEP with ab155421 antibody at 1/100.
Anti-ABCB11/BSEP antibody (ab155421) at 1/1000 dilution + Jurkat whole cell
lysate at 30 µg
Predicted band size: 146 kDa
5% SDS PAGE
Immunofluorescent analysis of methanol-fixed A431 cells labeling ABCB11/BSEP with ab155421 at 1/500. Image at the right costained with Hoechst 33342.
ab155421 has been referenced in 4 publications.
- Liu L et al. Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver-Gut Axis. Front Pharmacol 11:12 (2020). PubMed: 32116693
- Gan L et al. Functional analysis of the correlation between ABCB11 gene mutation and primary intrahepatic stone. Mol Med Rep N/A:N/A (2018). PubMed: 30431138
- Mather Q et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). PubMed: 28781809
- Zhou JJ et al. Knockdown of long non-coding RNA HOTAIR sensitizes hepatocellular carcinoma cell to cisplatin by suppressing the STAT3/ABCB1 signaling pathway. Oncol Lett 14:7986-7992 (2017). PubMed: 29250186