Key features and details
- Rabbit polyclonal to ABCB11/BSEP
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ABCB11/BSEP antibody
See all ABCB11/BSEP primary antibodies
DescriptionRabbit polyclonal to ABCB11/BSEP
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat pancreatic and human liver carcinoma tissues.
This product was previously labelled as ABCB11
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionInvolved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
Tissue specificityExpressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
Involvement in diseaseDefects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
- Information by UniProt
- ABC member 16, MDR/TAP subfamily antibody
- ABC16 antibody
- ABCB1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreatic tissue labeling ABCB11/BSEP with ab217532 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded human liver carcinoma tissue labeling ABCB11/BSEP with ab217532 at 1/500 dilution overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes and DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH 6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes.
ab217532 has been referenced in 2 publications.
- Chen YS et al. Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice. Cells 8:N/A (2019). PubMed: 30884843
- Lee S et al. Effects of Red Ginseng Extract on the Pharmacokinetics and Elimination of Methotrexate via Mrp2 Regulation. Molecules 23:N/A (2018). PubMed: 30424502