Overview

  • Product name

    Anti-ABCB4 antibody - N-terminal
    See all ABCB4 primary antibodies
  • Description

    Rabbit polyclonal to ABCB4 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Mouse ABCB4 aa 1-43 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P21447

  • Positive control

    • Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab202976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300.
IHC-P 1/100 - 1/500.

(or 1/50 - 1/200 if using a fluorescent secondary antibody).

Target

  • Function

    Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
  • Involvement in disease

    Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
    Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
    Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC 21 antibody
    • ABC B4 antibody
    • ABC21 antibody
    • ABCB 4 antibody
    • Abcb4 antibody
    • ABCB4 protein antibody
    • ATP binding cassette sub family B MDR/TAP member 4 antibody
    • ATP binding cassette sub family B member 4 antibody
    • ATP-binding cassette sub-family B member 4 antibody
    • GBD1 antibody
    • ICP3 antibody
    • MDR 3 antibody
    • MDR2 antibody
    • MDR2/3 antibody
    • MDR3 antibody
    • MDR3 P glycoprotein antibody
    • MDR3 P gp antibody
    • MDR3_HUMAN antibody
    • Multidrug resistance protein 3 antibody
    • Multiple drug resistance 3 antibody
    • P glycoprotein 3 antibody
    • P-glycoprotein 3 antibody
    • PFIC 3 antibody
    • PFIC3 antibody
    • PGY 3 antibody
    • PGY3 antibody
    see all

Images

  • Anti-ABCB4 antibody - N-terminal (ab202976) at 1/300 dilution + Rat liver lysates

    Secondary
    Conjugated secondary antibody at 1/10000 dilution
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling ABCB4 with ab202976 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab202976 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (5% gel, UNHEATED samples)
Loading amount
30 µg
Specification
Liver

Asmita Bhattacharya

Verified customer

Submitted Feb 14 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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