• Product name
    Anti-ABCB4 antibody [P3II-26]
    See all ABCB4 primary antibodies
  • Description
    Mouse monoclonal [P3II-26] to ABCB4
  • Host species
  • Specificity
    Clone P3II-26 does not cross-react with the human ABCB1.
  • Tested applications
    Suitable for: ICC, IHC-Fr, WB, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein:


    , corresponding to Internal sequence amino acids 629-692 of Human ABCB4.

  • Epitope
    Clone P3II-26 reacts with an internal epitope of ABCB4.



Our Abpromise guarantee covers the use of ab24108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/20 - 1/50. Fix with acetone.
IHC-Fr 1/20. Fix with acetone.
WB Use at an assay dependent concentration. Predicted molecular weight: 141 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
    • Involvement in disease
      Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
      Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
      Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.
    • Sequence similarities
      Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC 21 antibody
      • ABC B4 antibody
      • ABC21 antibody
      • ABCB 4 antibody
      • Abcb4 antibody
      • ABCB4 protein antibody
      • ATP binding cassette sub family B MDR/TAP member 4 antibody
      • ATP binding cassette sub family B member 4 antibody
      • ATP-binding cassette sub-family B member 4 antibody
      • GBD1 antibody
      • ICP3 antibody
      • MDR 3 antibody
      • MDR2 antibody
      • MDR2/3 antibody
      • MDR3 antibody
      • MDR3 P glycoprotein antibody
      • MDR3 P gp antibody
      • MDR3_HUMAN antibody
      • Multidrug resistance protein 3 antibody
      • Multiple drug resistance 3 antibody
      • P glycoprotein 3 antibody
      • P-glycoprotein 3 antibody
      • PFIC 3 antibody
      • PFIC3 antibody
      • PGY 3 antibody
      • PGY3 antibody
      see all


    • Overlay histogram showing HeLa cells stained with ab24108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24108, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


    This product has been referenced in:
    • Gordillo GM  et al. Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells. J Biol Chem 291:10089-103 (2016). Read more (PubMed: 26961872) »
    • Scheffer GL  et al. Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies. Cancer Res 60:5269-77 (2000). Read more (PubMed: 11016657) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the datasheets of these antibodies and select a product according to your requirements. The anti MDR2 products are ab71792; ab24108 and ab111209 Anti MRP1 products are ab24102; ab63987; ab99531; ab81903 To search different products please type target name in the search box provided on the Abcam homepage at www.abcam.com. After hitting the search button the system will give you the sorted product as per your target request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Thank you for your enquiry. This antibody was tested in Western blot. I could not find a specific dilution recommended for this antibody, but in general we recommend using 1-20ug/ml of primary antibody, and this antibody is supplied as approximately 250 µg immunoglobulin/ml. Therefore, I would recommend starting at 1:100 and optimizing from there. I would recommend liver tissue as a positive control, since the datasheet states that this protein mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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