Key features and details
- Mouse monoclonal [P3II-26] to ABCB4
- Suitable for: ICC, IHC-Fr, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-ABCB4 antibody [P3II-26]
See all ABCB4 primary antibodies
DescriptionMouse monoclonal [P3II-26] to ABCB4
SpecificityClone P3II-26 does not cross-react with the human ABCB1.
Tested applicationsSuitable for: ICC, IHC-Fr, WB, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
EpitopeClone P3II-26 reacts with an internal epitope of ABCB4.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.7% BSA
Serum free tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab24108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/20 - 1/50. Fix with acetone.|
|IHC-Fr||1/20. Fix with acetone.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 141 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionMediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
Involvement in diseaseDefects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
Cellular localizationCell membrane.
- Information by UniProt
- ABC 21 antibody
- ABC B4 antibody
- ABC21 antibody
Overlay histogram showing HeLa cells stained with ab24108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24108, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab24108 has been referenced in 3 publications.
- Wen C et al. Curcumin reverses doxorubicin resistance via inhibition the efflux function of ABCB4 in doxorubicin-resistant breast cancer cells. Mol Med Rep 19:5162-5168 (2019). PubMed: 31059026
- Gordillo GM et al. Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells. J Biol Chem 291:10089-103 (2016). PubMed: 26961872
- Scheffer GL et al. Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies. Cancer Res 60:5269-77 (2000). PubMed: 11016657