Overview

  • Product name

    Anti-ABCB5 antibody [5H3C6]
    See all ABCB5 primary antibodies
  • Description

    Mouse monoclonal [5H3C6] to ABCB5
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment, corresponding to amino acids 481-674 of Human ABCB5, expressed in E. Coli.

  • Positive control

    • Recombinant Human ABCB5 protein; A431 cell lysate; HepG2 cells; Human liver cancer and breast cancer tissues.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR206345-20 are from Tissue Culture Supernatant

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabilizer.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    5H3C6
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 90 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas.
  • Tissue specificity

    Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABCB 5 antibody
    • ABCB5 antibody
    • ABCB5 P-gp antibody
    • ABCB5_HUMAN antibody
    • ABCB5alpha antibody
    • ABCB5beta antibody
    • ATP binding cassette protein antibody
    • ATP binding cassette sub family B (MDR/TAP) member 5 antibody
    • ATP binding cassette sub family B member 5 antibody
    • ATP-binding cassette sub-family B member 5 antibody
    • EST422562 antibody
    • P glycoprotein ABCB5 antibody
    • P-glycoprotein ABCB5 antibody
    see all

Images

  • Flow cytometric analysis of HepG2 cells labeling ABCB5 with ab140667 at 1/200 dilution (green);negative control (red).
  • Immunofluorescent analysis of HepG2 cells labeling ABCB5 with ab140667 at 1/200 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Anti-ABCB5 antibody [5H3C6] (ab140667) at 1/500 dilution + A431 cell lysate.

    Predicted band size: 90 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling ABCB5 with ab140667 at 1/200 dilution followed by DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ABCB5 with ab140667 at 1/200 dilution followed by DAB staining.
  • ELISA analysis using ab140667. Antigen = Purified recombinant fragment, corresponding to amino acids 481-674 of Human ABCB5, expressed in E. Coli.

References

This product has been referenced in:

  • Xiao J  et al. Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines. BMC Cancer 18:675 (2018). Read more (PubMed: 29929490) »
  • Wang S  et al. ABCB5 promotes melanoma metastasis through enhancing NF-?B p65 protein stability. Biochem Biophys Res Commun 492:18-26 (2017). WB . Read more (PubMed: 28821433) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Corneal limbus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA (ph 9.0)
Permeabilization
Yes - PBT: 0.1% Triton X-100 in PBS
Specification
Corneal limbus
Blocking step
10% FBS + 5% BSA in PBT as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Noelia Andollo

Verified customer

Submitted Oct 08 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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