Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ABCB5 antibody [5H3C6]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Corneal limbus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA (ph 9.0)
Permeabilization
Yes - PBT: 0.1% Triton X-100 in PBS
Specification
Corneal limbus
Blocking step
10% FBS + 5% BSA in PBT as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG1
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568

Additional data

Additional Notes
Very specific staining: only some cells in the limbal area where limbal crypts are described.

Dr. Noelia Andollo

Verified customer

Submitted Oct 08 2018

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