Recombinant
RabMAb

Recombinant Anti-ABCB6/PRP antibody [EPR21891] - BSA and Azide free (ab236457)

Overview

  • Product name

    Anti-ABCB6/PRP antibody [EPR21891] - BSA and Azide free
    See all ABCB6/PRP primary antibodies
  • Description

    Rabbit monoclonal [EPR21891] to ABCB6/PRP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ABCB6/PRP aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NP58

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    ab236457 is the carrier-free version of ab221159 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236457 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ABCB6

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Binds heme and porphyrins and functions in their ATP-dependent uptake into the mitochondria. Plays a crucial role in heme synthesis.
  • Tissue specificity

    Widely expressed. Highest expression in heart and skeletal muscles.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • Developmental stage

    Highly expressed in fetal liver.
  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC antibody
    • ABC transporter umat antibody
    • ABC14 antibody
    • Abcb6 antibody
    • ABCB6_HUMAN antibody
    • ATP binding cassette sub family B member 6, mitochondrial precursor antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 6 antibody
    • ATP-binding cassette half-transporter antibody
    • ATP-binding cassette sub-family B member 6 antibody
    • ATP-binding cassette sub-family B member 6, mitochondrial antibody
    • DUH3 antibody
    • LAN antibody
    • MCOPCB7 antibody
    • Mitochondrial ABC transporter 3 antibody
    • mitochondrial antibody
    • Mt ABC transporter 3 antibody
    • Mt-ABC transporter 3 antibody
    • MTABC3 antibody
    • P glycoprotein related protein antibody
    • P-glycoprotein-related protein antibody
    • PRP antibody
    • Ubiquitously expressed mammalian ABC half transporter antibody
    • Ubiquitously-expressed mammalian ABC half transporter antibody
    • UMAT antibody
    see all

Images

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ABCB6/PRP with ab221159 at 1/500 dilution (red)compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221159).

  • Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling ABCB6/PRP with ab221159 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing lysosome staining in K-562 cell line.

    The nuclear counter stain is DAPI (blue). Counterstained with ab25630 anti-LAMP1 (mouse mAb) at 100 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221159).

  • Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human  epithelial cell line from cervix adenocarcinoma) cells labeling ABCB6/PRP with ab221159 at 1/500 dilution, followed by ab150077 AlexaFluorGoat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing lysosome staining in K-562 cell line.

    The nuclear counter stain is DAPI (blue). Counterstained with ab25630 anti-LAMP1 (mouse mAb) at 100 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221159).

References

ab236457 has not yet been referenced specifically in any publications.

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