The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human ABCB7 peptide (ab66358).
Could be involved in the transport of heme from the mitochondria to the cytosol. Plays a central role in the maturation of cytosolic iron-sulfur (Fe/S) cluster-containing proteins.
Involvement in disease
Defects in ABCB7 are the cause of X-linked sideroblastic anemia with ataxia (ASAT) [MIM:301310]. ASAT is a recessive disorder characterized by an infantile to early childhood onset of nonprogressive cerebellar ataxia and mild anemia with hypochromia and microcytosis.
Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.
ATP binding cassette sub family B (MDR/TAP) member 7 antibody
ATP binding cassette sub family B member 7 antibody
ATP binding cassette sub family B member 7 mitochondrial antibody
ATP binding cassette transporter 7 antibody
ATP-binding cassette sub-family B member 7 antibody
ATP-binding cassette transporter 7 antibody
Multidrug resistance protein 7 antibody
P-glycoprotein 7 antibody
Western blot - Anti-ABCB7 antibody (ab116606)
All lanes : Anti-ABCB7 antibody (ab116606) at 1 µg/ml
Lane 1 : Thymus (Rat) Tissue Lysate Lane 2 : Human spinal cord tissue lysate - total protein (ab29188) Lane 3 : Human kidney tissue lysate - total protein (ab30203) Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa Observed band size: 83 kDa
Exposure time: 90 seconds
Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.