• Product name
  • Description
    Rabbit polyclonal to ABCB9
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 475 and 525 of human ABCB9 (NP_062571.1)

  • Positive control
    • HeLa, 293T and mouse NIH3T3 whole cell lysates



Our Abpromise guarantee covers the use of ab86222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Relevance
    The membrane associated protein encoded by this gene is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in three known products which are likely to have different substrate specifications.
  • Cellular localization
    Integral membrane protein. Lysosomal. May be localized in the endoplasmic reticulum.
  • Database links
  • Alternative names
    • ABC transporter 9 protein antibody
    • ABCB 9 antibody
    • ATP binding cassette sub family B (MDR/TAP) member 9 antibody
    • ATP binding cassette sub family B member 9 precursor antibody
    • ATP binding cassette transporter 9 antibody
    • EST122234 antibody
    • hABCB9 antibody
    • KIAA1520 antibody
    • TAP like protein antibody
    • TAPL antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ABCB9 with ab86222 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ABCB9 antibody (ab86222) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 84 kDa

    Exposure time: 10 seconds
  • Detection of human ABCB9 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86222 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 3 seconds.


This product has been referenced in:
  • Rodriguez-Navarro JA  et al. Inhibitory effect of dietary lipids on chaperone-mediated autophagy. Proc Natl Acad Sci U S A 109:E705-14 (2012). WB ; Mouse . Read more (PubMed: 22331875) »

See 1 Publication for this product

Customer reviews and Q&As

Unfortunately, no we do not have the blocking peptide commercially available for this product. I apologize for the inconvenience.

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The antibody ab45371 is currently out of stock until 14th January. The items will be dispatched on 15th January with order number 1233746.

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Thank very much for your patience while laboratory was retesting the specificity of this antibody.

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