Overview

  • Product name

  • Description

    Rabbit polyclonal to ABCE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ABCE1 (internal sequence). The exact sequence is proprietary.
    Database link: P61221

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human colon carcinoma tissue. WB: K562, Jurkat, Raji and NCI-H929 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 67 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCE family.
    Contains 2 4Fe-4S ferredoxin-type domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2' 5' oligoadenylate binding protein antibody
    • 2''-5''-oligoadenylate-binding protein antibody
    • 2'5' oligoadenylate binding protein antibody
    • ABC 38 antibody
    • ABC38 antibody
    • ABCE 1 antibody
    • ABCE1 antibody
    • ABCE1_HUMAN antibody
    • ATP binding cassette sub family E (OABP) member 1 antibody
    • ATP binding cassette sub family E member 1 antibody
    • ATP-binding cassette sub-family E member 1 antibody
    • HuHP 68 antibody
    • HuHP68 antibody
    • OABP antibody
    • Ribonuclease 4 inhibitor antibody
    • Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor antibody
    • Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor antibody
    • Ribonuclease L inhibitor antibody
    • RLI antibody
    • RNase L inhibitor antibody
    • RNASEL1 antibody
    • RNASELI antibody
    • RNS 4I antibody
    • RNS4I antibody
    see all

Images

  • All lanes : Anti-ABCE1 antibody (ab227464) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extracts
    Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 67 kDa



    7.5% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ABCE1 (green) using ab227464 (1/500 dilution) in ICC/IF. Cells were fixed in 2% paraformaldehyde/ culture medium at 37°C for 30 minutes.

    Counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded human colon carcinoma tissue stained for ABCE1 with ab227464 (1/500 dilution) in immunohistochemical analysis.

  • Anti-ABCE1 antibody (ab227464) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa



    7.5 % SDS-PAGE

References

ab227464 has not yet been referenced specifically in any publications.

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