Recombinant Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free
See all ABCE1 primary antibodies -
Description
Rabbit monoclonal [EPR15373(B)] to ABCE1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, Flow Cyt, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ABCE1 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P61221 -
General notes
Ab250835 is the carrier-free version of ab185548. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15373(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab250835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCE family.
Contains 2 4Fe-4S ferredoxin-type domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 6059 Human
- Entrez Gene: 24015 Mouse
- Entrez Gene: 361390 Rat
- Omim: 601213 Human
- SwissProt: P61221 Human
- SwissProt: P61222 Mouse
- Unigene: 12013 Human
- Unigene: 5831 Mouse
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Alternative names
- 2' 5' oligoadenylate binding protein antibody
- 2''-5''-oligoadenylate-binding protein antibody
- 2'5' oligoadenylate binding protein antibody
see all
Datasheets and documents
References
ab250835 has not yet been referenced specifically in any publications.
