Recombinant Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15373(B)] to ABCE1 - BSA and Azide free
- Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free
See all ABCE1 primary antibodies -
Description
Rabbit monoclonal [EPR15373(B)] to ABCE1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P MouseIP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250835 is the carrier-free version of ab185548. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15373(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IHC-P |
Mouse
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IP |
Human
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WB |
Human
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Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCE family.
Contains 2 4Fe-4S ferredoxin-type domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 6059 Human
- Entrez Gene: 24015 Mouse
- Entrez Gene: 361390 Rat
- Omim: 601213 Human
- SwissProt: P61221 Human
- SwissProt: P61222 Mouse
- Unigene: 12013 Human
- Unigene: 5831 Mouse
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Alternative names
- 2' 5' oligoadenylate binding protein antibody
- 2''-5''-oligoadenylate-binding protein antibody
- 2'5' oligoadenylate binding protein antibody
see all
Images
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All lanes : Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaThis data was developed using ab185548, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
This data was developed using ab185548, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)This data was developed using ab185548, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded mouse liver tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab185548, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of K562 cells labeling ABCE1 using ab185548 at a 1/220 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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This data was developed using ab185548, the same antibody clone in a different buffer formulation.Western blot analysis of ABCE1 in immunoprecipitation pellets from 293 lysate (Lane 1) or 1XPBS (negative control) (Lane 2) using ab185548 at 1/50 dilution. Secondary antibody, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab250835 has not yet been referenced specifically in any publications.