RecombinantRabMAb

Recombinant Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

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Western blot - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Flow Cytometry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
  • Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15373(B)] to ABCE1 - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free
    See all ABCE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15373(B)] to ABCE1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250835 is the carrier-free version of ab185548. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Mouse
IP
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

  • Function

    Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCE family.
    Contains 2 4Fe-4S ferredoxin-type domains.
    Contains 2 ABC transporter domains.

  • Cellular localization

    Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 2' 5' oligoadenylate binding protein antibody
      • 2''-5''-oligoadenylate-binding protein antibody
      • 2'5' oligoadenylate binding protein antibody
      • ABC 38 antibody
      • ABC38 antibody
      • ABCE 1 antibody
      • ABCE1 antibody
      • ABCE1_HUMAN antibody
      • ATP binding cassette sub family E (OABP) member 1 antibody
      • ATP binding cassette sub family E member 1 antibody
      • ATP-binding cassette sub-family E member 1 antibody
      • HuHP 68 antibody
      • HuHP68 antibody
      • OABP antibody
      • Ribonuclease 4 inhibitor antibody
      • Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor antibody
      • Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor antibody
      • Ribonuclease L inhibitor antibody
      • RLI antibody
      • RNase L inhibitor antibody
      • RNASEL1 antibody
      • RNASELI antibody
      • RNS 4I antibody
      • RNS4I antibody
      see all

    Images

    • Western blot - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Western blot - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      All lanes : Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548) at 1/5000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 67 kDa
      Observed band size: 67 kDa



      This data was developed using ab185548, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

      This data was developed using ab185548, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      This data was developed using ab185548, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded mouse liver tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      This data was developed using ab185548, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling ABCE1 using ab185548 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.
    • Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      This data was developed using ab185548, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of formaldehyde fixed 293 cells labeling ABCE1 using ab185548 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.
    • Flow Cytometry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Flow Cytometry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      This data was developed using ab185548, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of K562 cells labeling ABCE1 using ab185548 at a 1/220 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
    • Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      This data was developed using ab185548, the same antibody clone in a different buffer formulation.Western blot analysis of ABCE1 in immunoprecipitation pellets from 293 lysate (Lane 1) or 1XPBS (negative control) (Lane 2) using ab185548 at 1/50 dilution. Secondary antibody, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.
    • Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
      Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250835? Please let us know so that we can cite the reference in this datasheet.

    ab250835 has not yet been referenced specifically in any publications.

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