Recombinant
RabMAb

Recombinant Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

Overview

  • Product name

    Anti-ABCE1 antibody [EPR15373(B)] - C-terminal
    See all ABCE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCE1 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P61221

  • Positive control

    • 293, K562 and HeLa cell lysate. Human breast carcinoma. Mouse liver. HeLa and 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/30 - 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/100.
Flow Cyt 1/220.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCE family.
    Contains 2 4Fe-4S ferredoxin-type domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2' 5' oligoadenylate binding protein antibody
    • 2''-5''-oligoadenylate-binding protein antibody
    • 2'5' oligoadenylate binding protein antibody
    • ABC 38 antibody
    • ABC38 antibody
    • ABCE 1 antibody
    • ABCE1 antibody
    • ABCE1_HUMAN antibody
    • ATP binding cassette sub family E (OABP) member 1 antibody
    • ATP binding cassette sub family E member 1 antibody
    • ATP-binding cassette sub-family E member 1 antibody
    • HuHP 68 antibody
    • HuHP68 antibody
    • OABP antibody
    • Ribonuclease 4 inhibitor antibody
    • Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor antibody
    • Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor antibody
    • Ribonuclease L inhibitor antibody
    • RLI antibody
    • RNase L inhibitor antibody
    • RNASEL1 antibody
    • RNASELI antibody
    • RNS 4I antibody
    • RNS4I antibody
    see all

Images

  • All lanes : Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded mouse liver tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling ABCE1 using ab185548 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Immunofluorescent analysis of formaldehyde fixed 293 cells labeling ABCE1 using ab185548 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.

  • Flow Cytometry analysis of K562 cells labeling ABCE1 using ab185548 at a 1/220 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Western blot analysis of ABCE1 in immunoprecipitation pellets from 293 lysate (Lane 1) or 1XPBS (negative control) (Lane 2) using ab185548 at 1/50 dilution. Secondary antibody, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.

References

This product has been referenced in:

  • Liang Z  et al. Tip60-siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway. Exp Ther Med 17:3195-3202 (2019). Read more (PubMed: 30936993) »
  • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). Read more (PubMed: 30765471) »
See all 5 Publications for this product

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