Recombinant
RabMAb

Recombinant Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (HRP) (ab209043)

Overview

  • Product name

    Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (HRP)
    See all ABCE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCE1 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P61221

  • Positive control

    • WB: HEK293, K562, HeLa cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

  • Function

    Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCE family.
    Contains 2 4Fe-4S ferredoxin-type domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2' 5' oligoadenylate binding protein antibody
    • 2''-5''-oligoadenylate-binding protein antibody
    • 2'5' oligoadenylate binding protein antibody
    • ABC 38 antibody
    • ABC38 antibody
    • ABCE 1 antibody
    • ABCE1 antibody
    • ABCE1_HUMAN antibody
    • ATP binding cassette sub family E (OABP) member 1 antibody
    • ATP binding cassette sub family E member 1 antibody
    • ATP-binding cassette sub-family E member 1 antibody
    • HuHP 68 antibody
    • HuHP68 antibody
    • OABP antibody
    • Ribonuclease 4 inhibitor antibody
    • Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor antibody
    • Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor antibody
    • Ribonuclease L inhibitor antibody
    • RLI antibody
    • RNase L inhibitor antibody
    • RNASEL1 antibody
    • RNASELI antibody
    • RNS 4I antibody
    • RNS4I antibody
    see all

Images

  • All lanes : Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (HRP) (ab209043) at 1/5000 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209043 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209043 has not yet been referenced specifically in any publications.

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