Anti-ABCF1 antibody [ABC5H06] (ab50976)
Key features and details
- Mouse monoclonal [ABC5H06] to ABCF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ABCF1 antibody [ABC5H06]
See all ABCF1 primary antibodies -
Description
Mouse monoclonal [ABC5H06] to ABCF1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human ABCF1 fragment
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.316% Tris HCl, 1% BSA, 1.16% Sodium chloride -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Concentrated and dialyzed culture medium (protein free medium) of hybridoma -
Clonality
Monoclonal -
Clone number
ABC5H06 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/100. Detects a band of approximately 85 kDa (predicted molecular weight: 96 kDa).
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Notes |
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WB
1/100. Detects a band of approximately 85 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Contains 2 ABC transporter domains. -
Post-translational
modificationsIsoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes. -
Cellular localization
Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope. - Information by UniProt
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Database links
- Entrez Gene: 23 Human
- Omim: 603429 Human
- SwissProt: Q8NE71 Human
- Unigene: 655285 Human
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Alternative names
- ABC27 antibody
- ABC50 antibody
- ABCF1 antibody
see all
Images
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Anti-ABCF1 antibody [ABC5H06] (ab50976) at 1/100 dilution + RIPA lysate of HeLa cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 96 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab50976 has been referenced in 1 publication.
- Pechenino AS et al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB ; Rat . PubMed: 21750230