Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.316% Tris HCl, 1% BSA, 1.16% Sodium chloride
PurityTissue culture supernatant
Purification notesConcentrated and dialyzed culture medium (protein free medium) of hybridoma
Our Abpromise guarantee covers the use of ab50976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 85 kDa (predicted molecular weight: 96 kDa).|
|IP||Use at an assay dependent dilution.|
|Dot blot||Use at an assay dependent dilution.|
FunctionIsoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Contains 2 ABC transporter domains.
modificationsIsoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
- Information by UniProt
- ABC27 antibody
- ABC50 antibody
- ABCF1 antibody
Anti-ABCF1 antibody [ABC5H06] (ab50976) at 1/100 dilution + RIPA lysate of HeLa cells at 500 µg
Anti-mouse IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 96 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?