Key features and details
- Mouse monoclonal [ABC5H06] to ABCF1
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ABCF1 antibody [ABC5H06]
See all ABCF1 primary antibodies
DescriptionMouse monoclonal [ABC5H06] to ABCF1
Tested applicationsSuitable for: WB, IP, Dot blotmore details
Species reactivityReacts with: Human
Recombinant human ABCF1 fragment
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.316% Tris HCl, 1% BSA, 1.16% Sodium chloride
Concentration information loading...
PurityTissue culture supernatant
Purification notesConcentrated and dialyzed culture medium (protein free medium) of hybridoma
Our Abpromise guarantee covers the use of ab50976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 85 kDa (predicted molecular weight: 96 kDa).|
|IP||Use at an assay dependent dilution.|
|Dot blot||Use at an assay dependent dilution.|
FunctionIsoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Contains 2 ABC transporter domains.
modificationsIsoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
- Information by UniProt
- ABC27 antibody
- ABC50 antibody
- ABCF1 antibody
Anti-ABCF1 antibody [ABC5H06] (ab50976) at 1/100 dilution + RIPA lysate of HeLa cells at 500 µg
Anti-mouse IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 96 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
ab50976 has been referenced in 1 publication.
- Pechenino AS et al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB ; Rat . PubMed: 21750230