Key features and details
- Rabbit polyclonal to ABCF2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ABCF2 antibody
See all ABCF2 primary antibodies
DescriptionRabbit polyclonal to ABCF2
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human ABCF2 aa 573-623. The exact sequence is proprietary. (NP_009120.1).
Database link: Q9UG63
- WB: HeLa and HEK-293T whole cell lysates. IHC-P: Human prostate carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab246011 was affinity purified using an epitope specific to ABCF2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab246011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/1000. Predicted molecular weight: 71 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceABCF 2 is a member of the ABC Transporter superfamily and of the GCN20 subfamily. As a member of the ABC Transporter family, it functions to transport various molecules across extra- and intracellular membranes.
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All lanes : Anti-ABCF2 antibody (ab246011) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ABCF2 using ab246011 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246011 has not yet been referenced specifically in any publications.