Anti-ABCG1 antibody [EP1366Y] (ab52617)
- Datasheet
- References (26)
- Protocols
Overview
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Product nameAnti-ABCG1 antibody [EP1366Y]
See all ABCG1 primary antibodies -
DescriptionRabbit monoclonal [EP1366Y] to ABCG1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Cow, Human
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Immunogen
Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.
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Positive control
- HUVEC cell lysate, human cervical carcinoma.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP1366Y
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IsotypeIgG
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Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab52617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa). |
Target
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FunctionTransporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
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Tissue specificityExpressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
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Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
- Information by UniProt
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Database links
- Entrez Gene: 510745 Cow
- Entrez Gene: 9619 Human
- Entrez Gene: 11307 Mouse
- Entrez Gene: 85264 Rat
- Omim: 603076 Human
- SwissProt: P45844 Human
- SwissProt: Q64343 Mouse
- Unigene: 124649 Human
see all -
Alternative names
- ABC transporter 8 antibody
- ABC8 antibody
- ABCG1 antibody
see all
Images
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![Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617-270913-ab52617WB1.jpg)
Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 20 µg
Lane 1 : Rat kidney lysate
Lane 2 : Rat liver lysate
Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 75 kDaBlocking and diluting buffer: 5% NFDM /TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617-270915-ab52617ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.
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![Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617wb.jpg)
Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antiibody at 1/2000 dilution
Predicted band size: 75 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.
References
This product has been referenced in:
- Zhao ZW et al. Heat shock protein 70 accelerates atherosclerosis by downregulating the expression of ABCA1 and ABCG1 through the JNK/Elk-1 pathway. Biochim Biophys Acta 1863:806-822 (2018). ICC/IF . Read more (PubMed: 29678642) »
- Hu J et al. Silencing ZAP70 prevents HSP65-induced reverse cholesterol transport and NF-?B activation in T cells. Biomed Pharmacother 102:271-277 (2018). Read more (PubMed: 29567540) »
