Recombinant Anti-ABCG1 antibody [EP1366Y] (ab52617)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1366Y] to ABCG1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human
Overview
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Product name
Anti-ABCG1 antibody [EP1366Y]
See all ABCG1 primary antibodies -
Description
Rabbit monoclonal [EP1366Y] to ABCG1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.
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Positive control
- HUVEC cell lysate, human cervical carcinoma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1366Y -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab52617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa). |
Target
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Function
Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. -
Tissue specificity
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes. - Information by UniProt
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Database links
- Entrez Gene: 510745 Cow
- Entrez Gene: 9619 Human
- Entrez Gene: 11307 Mouse
- Entrez Gene: 85264 Rat
- Omim: 603076 Human
- SwissProt: P45844 Human
- SwissProt: Q64343 Mouse
- Unigene: 124649 Human
see all -
Alternative names
- ABC transporter 8 antibody
- ABC8 antibody
- ABCG1 antibody
see all
Images
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![Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617-270913-anti-abcg1-antibody-ep1366y-western-blot.jpg)
Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 20 µg
Lane 1 : Rat kidney lysate
Lane 2 : Rat liver lysate
Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 75 kDaBlocking and diluting buffer: 5% NFDM /TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617-270915-anti-abcg1-antibody-ep1366y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.
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![Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617-64963-anti-abcg1-antibody-ep1366y-western-blot.jpg)
Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antiibody at 1/2000 dilution
Predicted band size: 75 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)](https://www.abcam.com/ps/products/52/ab52617/Images/ab52617-64962-anti-abcg1-antibody-ep1366y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.
References
ab52617 has been referenced in 42 publications.
- Liu J et al. ZAP70 deficiency promotes reverse cholesterol transport through MAPK/ERK pathway in Jurkat cell. Mol Immunol 107:21-28 (2019). PubMed: 30639475
- Storti F et al. Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration. Elife 8:N/A (2019). PubMed: 30864945
- Cai C et al. LncRNA ENST00000602558.1 regulates ABCG1 expression and cholesterol efflux from vascular smooth muscle cells through a p65-dependent pathway. Atherosclerosis 285:31-39 (2019). PubMed: 31003090
- Yang Z et al. Fucoidan A2 from the Brown Seaweed Ascophyllum nodosum Lowers Lipid by Improving Reverse Cholesterol Transport in C57BL/6J Mice Fed a High-Fat Diet. J Agric Food Chem 67:5782-5791 (2019). PubMed: 31055921
- Yin J et al. The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice. Food Funct 10:5124-5139 (2019). PubMed: 31364648
