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  1. Link

    abcg1-antibody-ep1366y-ab52617.pdf

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RecombinantRabMAb

Recombinant Anti-ABCG1 antibody [EP1366Y] (ab52617)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (67)

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Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)
  • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
  • Anti-ABCG1 antibody [EP1366Y] (ab52617)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1366Y] to ABCG1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Cow, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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Protein
Product image
Recombinant Human ABCG1 protein (ab153003)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-SREBP1 antibody (ab28481)

View more associated products

Overview

  • Product name

    Anti-ABCG1 antibody [EP1366Y]
    See all ABCG1 primary antibodies
  • Description

    Rabbit monoclonal [EP1366Y] to ABCG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HUVEC cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1366Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • HRP Anti-ABCG1 antibody [EP1366Y] (ab201776)
    • Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human ABCG1 protein (ab153003)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/100.
WB (3)
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
Notes
ICC/IF
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
  • Tissue specificity

    Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
  • Target information above from: UniProt accession P45844 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 510745 Cow
    • Entrez Gene: 9619 Human
    • Entrez Gene: 11307 Mouse
    • Entrez Gene: 85264 Rat
    • Omim: 603076 Human
    • SwissProt: P45844 Human
    • SwissProt: Q64343 Mouse
    • Unigene: 124649 Human
    • Unigene: 15691 Mouse
    • Unigene: 470747 Mouse
    see all
  • Alternative names

    • ABC transporter 8 antibody
    • ABC8 antibody
    • ABCG1 antibody
    • ABCG1_HUMAN antibody
    • ATP-binding cassette sub family G member 1 antibody
    • ATP-binding cassette sub-family G member 1 antibody
    • ATP-binding cassette transporter 8 antibody
    • ATP-binding cassette transporter member 1 of subfamily G antibody
    • ATP-binding cassette, sub family G WHITE member 1 antibody
    • homolog of Drosophila white antibody
    • MGC34313 antibody
    • White protein homolog antibody
    • White protein homolog ATP binding cassette transporter 8 antibody
    • WHITE1 antibody
    • WHT1 antibody
    see all

Images

  • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/1000 dilution ((purified))

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat liver lysate
    Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
    Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 75 kDa



    Blocking and diluting buffer: 5% NFDM /TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)

    ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

  • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antiibody at 1/2000 dilution

    Predicted band size: 75 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?

  • Anti-ABCG1 antibody [EP1366Y] (ab52617)
    Anti-ABCG1 antibody [EP1366Y] (ab52617)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (67)

Publishing research using ab52617? Please let us know so that we can cite the reference in this datasheet.

ab52617 has been referenced in 67 publications.

  • Yin Q  et al. Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis. J Cell Mol Med 25:5238-5249 (2021). PubMed: 33951300
  • Palmer MA  et al. Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle. Histochem Cell Biol 155:529-545 (2021). PubMed: 33404706
  • Zhang Z  et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
  • Lv JJ  et al. Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis. Front Cell Dev Biol 8:609090 (2020). PubMed: 33490072
  • Wang Y  et al. 27-Hydroxycholesterol Promotes the Transfer of Astrocyte-Derived Cholesterol to Neurons in Co-cultured SH-SY5Y Cells and C6 Cells. Front Cell Dev Biol 8:580599 (2020). PubMed: 33330456
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Synovial membrane)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Synovial membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 13 2016

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ABCG1 antibody [EP1366Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1M PBST with 10%donkeys serum
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 12 2016

Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brain lysate, Liver lysate and human serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ruma Raha-Chowdhury

Verified customer

Submitted Jan 28 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCG1 antibody [EP1366Y]

Poor
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2)
Specification
HepG2
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 13 2008

Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (endothelial cells from cerebral capillaries)
Loading amount
20 µg
Specification
endothelial cells from cerebral capillaries
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Fabien Gosselet

Verified customer

Submitted Mar 20 2008

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