Overview

  • Product name
    Anti-ABCG1 antibody [EP1366Y]
    See all ABCG1 primary antibodies
  • Description
    Rabbit monoclonal [EP1366Y] to ABCG1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • HUVEC cell lysate, human cervical carcinoma.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab52617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
    • Tissue specificity
      Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
    • Sequence similarities
      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • Cellular localization
      Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • ABC transporter 8 antibody
      • ABC8 antibody
      • ABCG1 antibody
      • ABCG1_HUMAN antibody
      • ATP-binding cassette sub family G member 1 antibody
      • ATP-binding cassette sub-family G member 1 antibody
      • ATP-binding cassette transporter 8 antibody
      • ATP-binding cassette transporter member 1 of subfamily G antibody
      • ATP-binding cassette, sub family G WHITE member 1 antibody
      • homolog of Drosophila white antibody
      • MGC34313 antibody
      • White protein homolog antibody
      • White protein homolog ATP binding cassette transporter 8 antibody
      • WHITE1 antibody
      • WHT1 antibody
      see all

    Images

    • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 20 µg

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat liver lysate
      Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
      Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
      Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

      Predicted band size: 75 kDa



      Blocking and diluting buffer: 5% NFDM /TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)

      ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

    • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP antiibody at 1/2000 dilution

      Predicted band size: 75 kDa
      Observed band size: 110 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.

    References

    This product has been referenced in:
    • Wen G  et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29437605) »
    • Zhao ZW  et al. Heat shock protein 70 accelerates atherosclerosis by downregulating the expression of ABCA1 and ABCG1 through the JNK/Elk-1 pathway. Biochim Biophys Acta 1863:806-822 (2018). ICC/IF . Read more (PubMed: 29678642) »

    See all 22 Publications for this product

    Publishing research using ab52617? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    5 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Synovial membrane)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    35 µg
    Specification
    Synovial membrane
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jun 13 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ABCG1 antibody [EP1366Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1M PBST with 10%donkeys serum
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 12 2016

    Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain lysate, Liver lysate and human serum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    abreview image
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jan 28 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCG1 antibody [EP1366Y]

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2)
    Specification
    HepG2
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Oct 13 2008

    Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (endothelial cells from cerebral capillaries)
    Loading amount
    20 µg
    Specification
    endothelial cells from cerebral capillaries
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    abreview image
    Username

    Dr. GOSSELET Fabien

    Verified customer

    Submitted Mar 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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