RecombinantRabMAb

Recombinant Anti-ABCG1 antibody [EP1366Y] (ab52617)

Reviews (5) Submit a question References (49)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1366Y] to ABCG1
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Cow, Human

Overview

  • Product name

    Anti-ABCG1 antibody [EP1366Y]
    See all ABCG1 primary antibodies

  • Description

    Rabbit monoclonal [EP1366Y] to ABCG1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Rat, Cow, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HUVEC cell lysate, human cervical carcinoma.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab52617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.

    • Tissue specificity

      Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.

    • Cellular localization

      Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ABC transporter 8 antibody
      • ABC8 antibody
      • ABCG1 antibody
      • ABCG1_HUMAN antibody
      • ATP-binding cassette sub family G member 1 antibody
      • ATP-binding cassette sub-family G member 1 antibody
      • ATP-binding cassette transporter 8 antibody
      • ATP-binding cassette transporter member 1 of subfamily G antibody
      • ATP-binding cassette, sub family G WHITE member 1 antibody
      • homolog of Drosophila white antibody
      • MGC34313 antibody
      • White protein homolog antibody
      • White protein homolog ATP binding cassette transporter 8 antibody
      • WHITE1 antibody
      • WHT1 antibody
      see all

    Images

    • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 20 µg

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat liver lysate
      Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
      Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
      Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

      Predicted band size: 75 kDa



      Blocking and diluting buffer: 5% NFDM /TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (ab52617)

      ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

    • Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP antiibody at 1/2000 dilution

      Predicted band size: 75 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.
    • Anti-ABCG1 antibody [EP1366Y] (ab52617)
      Anti-ABCG1 antibody [EP1366Y] (ab52617)

    References (49)

    Publishing research using ab52617? Please let us know so that we can cite the reference in this datasheet.

    ab52617 has been referenced in 49 publications.

    • Khamis A  et al. Histone deacetylase 9 promoter hypomethylation associated with adipocyte dysfunction is a statin-related metabolic effect. Clin Epigenetics 12:68 (2020). PubMed: 32410704
    • Ou M  et al. Long non-coding RNA CDKN2B-AS1 contributes to atherosclerotic plaque formation by forming RNA-DNA triplex in the CDKN2B promoter. EBioMedicine 55:102694 (2020). PubMed: 32335370
    • Li T  et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
    • Zhang F  et al. Inhibition of USP14 suppresses the formation of foam cell by promoting CD36 degradation. J Cell Mol Med 24:3292-3302 (2020). PubMed: 31970862
    • Gu X  et al. An in vitro model of foam cell formation induced by a stretchable microfluidic device. Sci Rep 9:7461 (2019). PubMed: 31097769
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Synovial membrane)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    35 µg
    Specification
    Synovial membrane
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 13 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ABCG1 antibody [EP1366Y]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1M PBST with 10%donkeys serum
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 12 2016

    Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain lysate, Liver lysate and human serum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jan 28 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCG1 antibody [EP1366Y]

     Poor
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2)
    Specification
    HepG2
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 13 2008

    Western blot abreview for Anti-ABCG1 antibody [EP1366Y]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (endothelial cells from cerebral capillaries)
    Loading amount
    20 µg
    Specification
    endothelial cells from cerebral capillaries
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Fabien Gosselet

    Verified customer

    Submitted Mar 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com