Recombinant
RabMAb

Recombinant Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)

Overview

  • Product name

    Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free
    See all ABCG1 primary antibodies
  • Description

    Rabbit monoclonal [EP1366Y] to ABCG1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    A synthetic peptide corresponding to residues on human ABCG1.

  • Positive control

    • HUVEC cell lysate, human cervical carcinoma.
  • General notes

    Ab218528 is the carrier-free version of ab52617. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab218528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab218528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
    • Tissue specificity

      Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • Cellular localization

      Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
    • Information by UniProt
    • Database links

    • Alternative names

      • ABC transporter 8 antibody
      • ABC8 antibody
      • ABCG1 antibody
      • ABCG1_HUMAN antibody
      • ATP-binding cassette sub family G member 1 antibody
      • ATP-binding cassette sub-family G member 1 antibody
      • ATP-binding cassette transporter 8 antibody
      • ATP-binding cassette transporter member 1 of subfamily G antibody
      • ATP-binding cassette, sub family G WHITE member 1 antibody
      • homolog of Drosophila white antibody
      • MGC34313 antibody
      • White protein homolog antibody
      • White protein homolog ATP binding cassette transporter 8 antibody
      • WHITE1 antibody
      • WHT1 antibody
      see all

    Images

    • ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52617).

    • Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52617).

    References

    This product has been referenced in:

    • Lin YW  et al. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages. J Mol Cell Cardiol 79:287-94 (2015). WB ; Mouse . Read more (PubMed: 25528964) »
    • Hu YW  et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res 55:681-97 (2014). Read more (PubMed: 24493833) »
    See all 13 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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