Overview

  • Product name

    Anti-ABCG1 antibody [EP1366Y] (HRP)
    See all ABCG1 primary antibodies
  • Description

    Rabbit monoclonal [EP1366Y] to ABCG1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide within Human ABCG1 aa 150-250. The exact sequence is proprietary.
    Database link: P45844

  • Positive control

    • WB: HUVEC whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Applications

Our Abpromise guarantee covers the use of ab201776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).

Target

  • Function

    Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
  • Tissue specificity

    Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC transporter 8 antibody
    • ABC8 antibody
    • ABCG1 antibody
    • ABCG1_HUMAN antibody
    • ATP-binding cassette sub family G member 1 antibody
    • ATP-binding cassette sub-family G member 1 antibody
    • ATP-binding cassette transporter 8 antibody
    • ATP-binding cassette transporter member 1 of subfamily G antibody
    • ATP-binding cassette, sub family G WHITE member 1 antibody
    • homolog of Drosophila white antibody
    • MGC34313 antibody
    • White protein homolog antibody
    • White protein homolog ATP binding cassette transporter 8 antibody
    • WHITE1 antibody
    • WHT1 antibody
    see all

Images

  • HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg with Anti-ABCG1 antibody [EP1366Y] (HRP) (ab201776) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 75 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201776 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

References

ab201776 has not yet been referenced specifically in any publications.

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