Recombinant Anti-ABCG5 antibody [EPR6203] (ab124965)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6203] to ABCG5
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ABCG5 antibody [EPR6203]
See all ABCG5 primary antibodies -
Description
Rabbit monoclonal [EPR6203] to ABCG5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ABCG5 aa 350-450. The exact sequence is proprietary.
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Positive control
- Ramos and HepG2 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 5.51 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6203 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab124965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). |
Target
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Function
Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. -
Tissue specificity
Strongly expressed in the liver, lower levels in the small intestine and colon. -
Involvement in disease
Defects in ABCG5 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 64240 Human
- Omim: 605459 Human
- SwissProt: Q9H222 Human
- Unigene: 132992 Human
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Alternative names
- Abcg5 antibody
- ABCG5_HUMAN antibody
- ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody
see all
Images
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All lanes : Anti-ABCG5 antibody [EPR6203] (ab124965) at 1/1000 dilution
Lane 1 : Ramos cell lysates
Lane 2 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
Datasheets and documents
References (0)
ab124965 has not yet been referenced specifically in any publications.