RecombinantRabMAb

Recombinant Anti-ABCG5 antibody [EPR6203] (ab124965)

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Western blot - Anti-ABCG5 antibody [EPR6203] (ab124965)
  • OI-RD Scanning - Anti-ABCG5 antibody [EPR6203] (ab124965)
  • Anti-ABCG5 antibody [EPR6203] (ab124965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6203] to ABCG5
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ABCG5 antibody [EPR6203]
    See all ABCG5 primary antibodies

  • Description

    Rabbit monoclonal [EPR6203] to ABCG5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ABCG5 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Ramos and HepG2 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab124965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

    • Tissue specificity

      Strongly expressed in the liver, lower levels in the small intestine and colon.

    • Involvement in disease

      Defects in ABCG5 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.

    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Abcg5 antibody
        • ABCG5_HUMAN antibody
        • ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody
        • ATP binding cassette sub family G member 5 antibody
        • ATP-binding cassette sub-family G member 5 antibody
        • ATP-binding cassette, sub-family G (WHITE), member 5 antibody
        • Sterolin 1 antibody
        • Sterolin-1 antibody
        • STSL antibody
        see all

      Images

      • Western blot - Anti-ABCG5 antibody [EPR6203] (ab124965)
        Western blot - Anti-ABCG5 antibody [EPR6203] (ab124965)
        All lanes : Anti-ABCG5 antibody [EPR6203] (ab124965) at 1/1000 dilution

        Lane 1 : Ramos cell lysates
        Lane 2 : HepG2 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 72 kDa

      • OI-RD Scanning - Anti-ABCG5 antibody [EPR6203] (ab124965)
        OI-RD Scanning - Anti-ABCG5 antibody [EPR6203] (ab124965)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-ABCG5 antibody [EPR6203] (ab124965)
        Anti-ABCG5 antibody [EPR6203] (ab124965)

      References (0)

      Publishing research using ab124965? Please let us know so that we can cite the reference in this datasheet.

      ab124965 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Inquiry: I'm interested in purchasing ABCG5 antibody that would cross react with rat tissue. There are no antibodies in your catalog that reacts with rat. Have these been tested with rat samples?Kindly advise me as to which one would be the best choice.

      Read More
      Answer

      I now have more information to share with you in regards to the immunogen used to raise the anti-ABCG5 antibody ab124965. As can be seen from the attachment, the immunogen used to raise this antibody also does not share high homology with the rat protein (76%). However, it is higher than either of the two other antibodies, ab69713 or ab87116.
      I would suggest the best option may be to try the antibody ab45279 as this has been raised against the full length protein. If you would like to try this I would be able to offer you a testing discount. This would involve you purchasing ab45279 as usual, testing it with your rat samples and sharing the results (regardless of whether positive or negative) through an Abreview. You would then be entitled to a free primary antibody of your choice (or the equivalent price of ab47279 off any product in our catalogue).
      More information on this offer can be found from the following link:
      www.abcam.com/collaborationdiscount
      If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab47279.
      I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

      Read More

      Question

      Inquiry: I'm interested in purchasing ABCG5 antibody that would cross react with rat tissue. There are no antibodies in your catalog that reacts with rat. Have these been tested with rat samples?Kindly advise me as to which one would be the best choice.

      Read More
      Answer

      Thank you for contacting us and your interest in our products.
      The ABCG5 antibodies which we have are all directed against the human protein. This shares 79% homology with the rat protein. We have not tested any of the anti-ABCG5 antibodies we have with rat samples, and we have had no reports of our customers having performed these experiments either. This does not mean that it would not work, simply that it has not been tested as yet.
      In the attached pdf you can find the sequence alignment of the human protein and rat protein as well as the immunogen sequence used to raise the anti-ABCG5 antibodies ab69713 and ab87116. As can be seen from this sequence alignment, these antibodies are very unlikely to be able to detect the rat protein as the homology within the immunogen region is very poor. You may have more luck in using the rabbit polyclonal ab45279 although I cannot guarantee this. I am currently seeking further information as to what the immunogen sequence used to raise ab124965 has been to see if this is more likely to work in your experiments.
      I hope this information has been of some help, I will let you know as soon as I have further information to share in regards to ab124965.

      Read More

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