- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-ABCG5 antibody [EPR6203]
See all ABCG5 primary antibodies -
DescriptionRabbit monoclonal [EPR6203] to ABCG5
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ABCG5 aa 350-450. The exact sequence is proprietary.
-
Positive control
- Ramos and HepG2 cell lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
-
Dissociation constant (KD)KD = 5.51 x 10 -10 M Learn more about KD
-
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR6203
-
IsotypeIgG
-
Research areas
Applications
Our Abpromise guarantee covers the use of ab124965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). |
Target
-
FunctionTransporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
-
Tissue specificityStrongly expressed in the liver, lower levels in the small intestine and colon.
-
Involvement in diseaseDefects in ABCG5 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
-
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Cellular localizationMembrane.
- Information by UniProt
-
Database links
- Entrez Gene: 64240 Human
- Omim: 605459 Human
- SwissProt: Q9H222 Human
- Unigene: 132992 Human
-
Alternative names
- Abcg5 antibody
- ABCG5_HUMAN antibody
- ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody
see all
Images
-
![Western blot - Anti-ABCG5 antibody [EPR6203] (ab124965)](https://www.abcam.com/ps/products/124/ab124965/Images/ABCG5-Primary-antibodies-ab124965-1.jpg)
Western blot - Anti-ABCG5 antibody [EPR6203] (ab124965)All lanes : Anti-ABCG5 antibody [EPR6203] (ab124965) at 1/1000 dilution
Lane 1 : Ramos cell lysates
Lane 2 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa -
![Other - Anti-ABCG5 antibody [EPR6203] (ab124965)](https://www.abcam.com/ps/products/124/ab124965/Images/ABCG5-Primary-antibodies-ab124965-2.jpg)
Other - Anti-ABCG5 antibody [EPR6203] (ab124965)
Protocols
References
ab124965 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"