Overview

  • Product name
  • Description
    Rabbit polyclonal to ABCG8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ABCG8 aa 176-413.
    Database link: Q9H221

  • Positive control
    • WB: Mouse heart, lung, kidney and stomach lysates. IHC-P: Human lung tissue. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function
    Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
  • Tissue specificity
    Strongly expressed in the liver, lower levels in the small intestine and colon. Detectable in a wide variety of human tissues.
  • Involvement in disease
    Genetic variations in ABCG8 can be associated with susceptibility to gallbladder disease type 4 (GBD4) [MIM:611465]. With an overall prevalence of 10-20%, gallstone disease (cholelithiasis) represents one of the most frequent and economically relevant health problems of industrialized countries.
    Defects in ABCG8 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCG8 antibody
    • ABCG8_HUMAN antibody
    • ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody
    • ATP binding cassette sub family G member 8 antibody
    • ATP binding cassette, sub family G (WHITE), member 8 antibody
    • ATP-binding cassette sub-family G member 8 antibody
    • GBD4 antibody
    • MGC142217 antibody
    • Sterolin 2 antibody
    • Sterolin-2 antibody
    • STSL antibody
    see all

Images

  • All lanes : Anti-ABCG8 antibody (ab223056) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse stomach lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 76 kDa
    Observed band size: 76 kDa

  • Paraffin-embedded human lung tissue stained for ABCG8 using ab223056 at 1/100 dilution in immunohistochemical analysis.

  • MCF7 (human breast adenocarcinoma cell line) cells stained for ABCG8 (green) using ab223056 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L).

References

ab223056 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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