Recombinant Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6177(2)] to ALKBH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ALKBH2 antibody [EPR6177(2)]
See all ALKBH2 primary antibodies -
Description
Rabbit monoclonal [EPR6177(2)] to ALKBH2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ALKBH2.
Database link: Q6NS38 -
Positive control
- Human fetal brain, Human testis, 293T, Mouse brain, Mouse heart, Rat brain, Rat heart Jurkat and HeLa lysates;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6177(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
Target
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Function
Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. -
Tissue specificity
Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. -
Sequence similarities
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Nucleus. Detected in replication foci during S-phase. - Information by UniProt
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Database links
- Entrez Gene: 121642 Human
- Entrez Gene: 231642 Mouse
- Entrez Gene: 304578 Rat
- Omim: 610602 Human
- SwissProt: Q6NS38 Human
- SwissProt: Q6P6J4 Mouse
- Unigene: 374458 Human
- Unigene: 332593 Mouse
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Alternative names
- 2OG Fe(II) oxy DC1 antibody
- 9530023G02 antibody
- ABH 2 antibody
see all
Images
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Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified)
Lane 1 : Human fetal brain lysates
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution
Lanes 1 & 3 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154859 has been referenced in 1 publication.
- Bonilla B et al. The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions. Elife 10:N/A (2021). PubMed: 34723799