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Anti-ABHD10 antibody (ab221630)

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Immunocytochemistry/ Immunofluorescence - Anti-ABHD10 antibody (ab221630)

    Key features and details

    • Rabbit polyclonal to ABHD10
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    Protein
    Product image
    Recombinant Human ABHD10 protein (ab126655)
    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-ABHD10 antibody
    • Description

      Rabbit polyclonal to ABHD10
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Orangutan
    • Immunogen

      Recombinant fragment corresponding to Human ABHD10 aa 125-205.
      Sequence:

      STLGKWRKDVLSIIDDLADGPQILVGSSLGGWLMLHAAIARPEKVVALIG VATAADTLVTKFNQLPVELKKEVEMKGVWSM


      Database link: Q9NUJ1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • ICC/IF: A431 cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human ABHD10 protein (ab126655)
    • Related Products

      • Recombinant Human ABHD10 protein (ab126655)

    Applications

    Our Abpromise guarantee covers the use of ab221630 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100.

    Target

    • Sequence similarities

      Belongs to the AB hydrolase superfamily.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession Q9NUJ1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 55347 Human
      • Entrez Gene: 100171612 Orangutan
      • SwissProt: Q9NUJ1 Human
      • SwissProt: Q5REX5 Orangutan
      • Unigene: 477115 Human
      • Unigene: 729137 Human
      • Alternative names

        • Abhd10 antibody
        • ABHDA_HUMAN antibody
        • Abhydrolase domain containing 10 antibody
        • Abhydrolase domain-containing protein 10 antibody
        • Abhydrolase domain-containing protein 10, mitochondrial antibody
        • Alpha/beta hydrolase domain containing protein 10 mitochondrial antibody
        • mitochondrial antibody
        • Mycophenolic acid acyl glucuronide esterase mitochondrial antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-ABHD10 antibody (ab221630)
        Immunocytochemistry/ Immunofluorescence - Anti-ABHD10 antibody (ab221630)

        PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for ABHD10 (green) using ab221630 at 4 µg/ml in ICC/IF.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab221630? Please let us know so that we can cite the reference in this datasheet.

      ab221630 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab221630.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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