Product nameAnti-ABHD12 antibody
See all ABHD12 primary antibodies
DescriptionMouse polyclonal to ABHD12
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length Human protein (NP_056415.1, aa 1 to ~404)
- ABHD12-transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
FunctionHas 2-arachidonoylglycerol hydrolase activity (By similarity). May be a regulator of endocannabinoid signaling pathways.
Involvement in diseaseDefects in ABHD12 are the cause of polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract (PHARC) [MIM:612674]. PHARC is a slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses.
Sequence similaritiesBelongs to the serine esterase family.
- Information by UniProt
- 2-arachidonoylglycerol hydrolase antibody
- 6330583M11Rik antibody
- ABD12_HUMAN antibody
All lanes : Anti-ABHD12 antibody (ab68949) at 1/500 dilution
Lane 1 : ABHD12 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
This product has been referenced in:
- Joshi A et al. Biochemical characterization of the PHARC-associated serine hydrolase ABHD12 reveals its preference for very-long-chain lipids. J Biol Chem 293:16953-16963 (2018). Read more (PubMed: 30237167) »
- Navia-Paldanius D et al. Biochemical and pharmacological characterization of human a/ß-hydrolase domain containing 6 (ABHD6) and 12 (ABHD12). J Lipid Res 53:2413-24 (2012). WB . Read more (PubMed: 22969151) »