Overview

  • Product name
  • Description
    Goat polyclonal to ABHD12
  • Host species
    Goat
  • Specificity
    ab81906 is expected to recognize isoform a (NP_001035937.1) only.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    C-REFLGKSEPEHQH

    , corresponding to C terminal amino acids 386-398 of Human ABHD12 (NP_001035937.1).

  • Positive control
    • Mouse Brain and Rat Brain lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab81906 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab81906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Has 2-arachidonoylglycerol hydrolase activity (By similarity). May be a regulator of endocannabinoid signaling pathways.
    • Involvement in disease
      Defects in ABHD12 are the cause of polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract (PHARC) [MIM:612674]. PHARC is a slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses.
    • Sequence similarities
      Belongs to the serine esterase family.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2-arachidonoylglycerol hydrolase antibody
      • 6330583M11Rik antibody
      • ABD12_HUMAN antibody
      • ABHD12 antibody
      • ABHD12A antibody
      • Abhydrolase domain containing protein 12 antibody
      • Abhydrolase domain-containing protein 12 antibody
      • AI431047 antibody
      • AW547313 antibody
      • BEM46L2 antibody
      • C20orf22 antibody
      • Monoacylglycerol lipase ABHD12 antibody
      • RP23-241M12.2 antibody
      see all

    Images

    • Anti-ABHD12 antibody (ab81906) at 0.3 µg/ml + Mouse Brain Lysate (in RIPA buffer) at 35 µg

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 40 kDa (why is the actual band size different from the predicted?)



      Primary incubation was 1 hour.

    References

    ab81906 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab81906.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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