Recombinant
RabMAb

Recombinant Anti-ABHD12 antibody [EPR13683] (ab180944)

Overview

  • Product name

    Anti-ABHD12 antibody [EPR13683]
    See all ABHD12 primary antibodies
  • Description

    Rabbit monoclonal [EPR13683] to ABHD12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ABHD12 aa 100-250. The exact sequence is proprietary.
    Database link: Q8N2K0

  • Positive control

    • SH-SY5Y cells. SH-SY5Y, U87-MG and THP-1 lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13683
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab180944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Has 2-arachidonoylglycerol hydrolase activity (By similarity). May be a regulator of endocannabinoid signaling pathways.
  • Involvement in disease

    Defects in ABHD12 are the cause of polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract (PHARC) [MIM:612674]. PHARC is a slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses.
  • Sequence similarities

    Belongs to the serine esterase family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2-arachidonoylglycerol hydrolase antibody
    • 6330583M11Rik antibody
    • ABD12_HUMAN antibody
    • ABHD12 antibody
    • ABHD12A antibody
    • Abhydrolase domain containing protein 12 antibody
    • Abhydrolase domain-containing protein 12 antibody
    • AI431047 antibody
    • AW547313 antibody
    • BEM46L2 antibody
    • C20orf22 antibody
    • Monoacylglycerol lipase ABHD12 antibody
    • RP23-241M12.2 antibody
    see all

Images

  • All lanes : Anti-ABHD12 antibody [EPR13683] (ab180944) at 1/5000 dilution

    Lane 1 : SH-SY5Y lysate
    Lane 2 : U87-MG lysate
    Lane 3 : THP-1 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Flow Cytometry analysis of SH-SY5Y cells labeling ABHD12 using ab180944 at a 1/170 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

References

ab180944 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180944.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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