Anti-ABHD14B antibody (ab235436)
Key features and details
- Rabbit polyclonal to ABHD14B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ABHD14B antibody
See all ABHD14B primary antibodies -
Description
Rabbit polyclonal to ABHD14B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human ABHD14B aa 1 to the C-terminus.
Database link: Q96IU4 -
Positive control
- WB: Mouse small intestine, spleen, liver and lung lysates. IHC-P: Human liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 22 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 22 kDa. |
Target
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Function
Has hydrolase activity towards p-nitrophenyl butyrate (in vitro). May activate transcription. -
Tissue specificity
Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. -
Sequence similarities
Belongs to the AB hydrolase superfamily. ABHD14 family. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 615289 Cow
- Entrez Gene: 84836 Human
- Entrez Gene: 76491 Mouse
- Entrez Gene: 100437476 Orangutan
- SwissProt: A7YY28 Cow
- SwissProt: Q96IU4 Human
- SwissProt: Q8VCR7 Mouse
- SwissProt: Q5R816 Orangutan
see all -
Alternative names
- ABHD14B antibody
- ABHEB_HUMAN antibody
- Abhydrolase domain containing 14B antibody
see all
Images
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All lanes : Anti-ABHD14B antibody (ab235436) at 1/500 dilution
Lane 1 : Mouse small intestine lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse lung lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 22 kDa -
Paraffin-embedded human liver tissue stained for ABHD14B using ab235436 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235436 has not yet been referenced specifically in any publications.